Adnectins: engineered target-binding protein therapeutics
D Lipovšek - Protein Engineering, Design & Selection, 2011 - academic.oup.com
AdnectinsTM are a new family of therapeutic proteins based on the 10th fibronectin type III
domain, and designed to bind with high affinity and specificity to therapeutically relevant …
domain, and designed to bind with high affinity and specificity to therapeutically relevant …
Beyond antibody engineering: directed evolution of alternative binding scaffolds and enzymes using yeast surface display
D Könning, H Kolmar - Microbial Cell Factories, 2018 - Springer
Pioneered exactly 20 years ago, yeast surface display (YSD) continues to take a major role
in protein engineering among the high-throughput display methodologies that have been …
in protein engineering among the high-throughput display methodologies that have been …
Picomolar affinity fibronectin domains engineered utilizing loop length diversity, recursive mutagenesis, and loop shuffling
BJ Hackel, A Kapila, KD Wittrup - Journal of molecular biology, 2008 - Elsevier
The 10th type III domain of human fibronectin (Fn3) has been validated as an effective
scaffold for molecular recognition. In the current work, it was desired to improve the …
scaffold for molecular recognition. In the current work, it was desired to improve the …
[HTML][HTML] Crystal structure of the complete integrin αVβ3 ectodomain plus an α/β transmembrane fragment
JP Xiong, B Mahalingham, JL Alonso… - The Journal of cell …, 2009 - ncbi.nlm.nih.gov
We determined the crystal structure of 1TM-αVβ3, which represents the complete
unconstrained ectodomain plus short C-terminal transmembrane stretches of the αV and β3 …
unconstrained ectodomain plus short C-terminal transmembrane stretches of the αV and β3 …
Structural basis for pure antagonism of integrin αVβ3 by a high-affinity form of fibronectin
JF Van Agthoven, JP Xiong, JL Alonso, X Rui… - Nature structural & …, 2014 - nature.com
Integrins are important therapeutic targets. However, current RGD-based anti-integrin drugs
are also partial agonists, inducing conformational changes that trigger potentially fatal …
are also partial agonists, inducing conformational changes that trigger potentially fatal …
Engineered cystine knot peptides that bind αvβ3, αvβ5, and α5β1 integrins with low‐nanomolar affinity
RH Kimura, AM Levin, FV Cochran… - Proteins: Structure …, 2009 - Wiley Online Library
There is a critical need for compounds that target cell surface integrin receptors for
applications in cancer therapy and diagnosis. We used directed evolution to engineer the …
applications in cancer therapy and diagnosis. We used directed evolution to engineer the …
Engineered cystine-knot peptides that bind αvβ3 integrin with antibody-like affinities
AP Silverman, AM Levin, JL Lahti… - Journal of molecular …, 2009 - Elsevier
The αvβ3 integrin receptor is an important cancer target due to its overexpression on many
solid tumors and the tumor neovasculature and its role in metastasis and angiogenesis. We …
solid tumors and the tumor neovasculature and its role in metastasis and angiogenesis. We …
Evolution of an interloop disulfide bond in high-affinity antibody mimics based on fibronectin type III domain and selected by yeast surface display: molecular …
D Lipovšek, SM Lippow, BJ Hackel… - Journal of molecular …, 2007 - Elsevier
The 10th human fibronectin type III domain (10Fn3) is one of several protein scaffolds used
to design and select families of proteins that bind with high affinity and specificity to …
to design and select families of proteins that bind with high affinity and specificity to …
FN3: a new protein scaffold reaches the clinic
L Bloom, V Calabro - Drug discovery today, 2009 - Elsevier
In the ten years since the first fibronectin type III (FN3) domain library was published, FN3
has continued to show promise as a scaffold for the generation of stable protein domains …
has continued to show promise as a scaffold for the generation of stable protein domains …
Antibody mimics based on human fibronectin type three domain engineered for thermostability and high-affinity binding to vascular endothelial growth factor receptor …
MH Parker, Y Chen, F Danehy, K Dufu… - Protein Engineering …, 2005 - academic.oup.com
The tenth human fibronectin type three domain (10Fn3) is a small (10 kDa), extremely stable
and soluble protein with an immunoglobulin-like fold, but without cysteine residues …
and soluble protein with an immunoglobulin-like fold, but without cysteine residues …