[HTML][HTML] CRISPR/Cas-mediated plant genome editing: outstanding challenges a decade after implementation

T Cardi, J Murovec, A Bakhsh, J Boniecka… - Trends in Plant …, 2023 - cell.com
The discovery of the CRISPR/Cas genome-editing system has revolutionized our
understanding of the plant genome. CRISPR/Cas has been used for over a decade to …

Beyond knockouts: fine‐tuning regulation of gene expression in plants with CRISPR‐Cas‐based promoter editing

X Tang, Y Zhang - New Phytologist, 2023 - Wiley Online Library
The CRISPR‐Cas‐based genome editing field in plants is expanding rapidly. Editing plant
promoters to obtain cis‐regulatory alleles with altered expression levels or patterns of target …

An efficient CRISPR–Cas12a promoter editing system for crop improvement

J Zhou, G Liu, Y Zhao, R Zhang, X Tang, L Li, X Jia… - Nature Plants, 2023 - nature.com
Promoter editing represents an innovative approach to introduce quantitative trait variation
(QTV) in crops. However, an efficient promoter editing system for QTV needs to be …

[HTML][HTML] Efficient plant genome engineering using a probiotic sourced CRISPR-Cas9 system

Z Zhong, G Liu, Z Tang, S Xiang, L Yang… - Nature …, 2023 - nature.com
Among CRISPR-Cas genome editing systems, Streptococcus pyogenes Cas9 (SpCas9),
sourced from a human pathogen, is the most widely used. Here, through in silico data …

Progress and prospect: Biosynthesis of plant natural products based on plant chassis

J Lin, X Yin, Y Zeng, X Hong, S Zhang, B Cui… - Biotechnology …, 2023 - Elsevier
Plant-derived natural products are a specific class of active substances with numerous
applications in the medical, energy, and industrial fields. Many of these substances are in …

[HTML][HTML] Strategies and methods for improving the efficiency of CRISPR/Cas9 gene editing in plant molecular breeding

J Zhou, X Luan, Y Liu, L Wang, J Wang, S Yang, S Liu… - Plants, 2023 - mdpi.com
Following recent developments and refinement, CRISPR-Cas9 gene-editing technology has
become increasingly mature and is being widely used for crop improvement. The application …

Enhancing the quality of staple food crops through CRISPR/Cas-mediated site-directed mutagenesis

OS Adeyinka, B Tabassum, BL Koloko, IV Ogungbe - Planta, 2023 - Springer
Main conclusion The enhancement of CRISPR-Cas gene editing with robust nuclease
activity promotes genetic modification of desirable agronomic traits, such as resistance to …

[HTML][HTML] High performance TadA-8e derived cytosine and dual base editors with undetectable off-target effects in plants

T Fan, Y Cheng, Y Wu, S Liu, X Tang, Y He… - Nature …, 2024 - nature.com
Cytosine base editors (CBEs) and adenine base editors (ABEs) enable precise C-to-T and A-
to-G edits. Recently, ABE8e, derived from TadA-8e, enhances A-to-G edits in mammalian …

[HTML][HTML] CRISPR technology towards genome editing of the perennial and semi-perennial crops citrus, coffee and sugarcane

GS Prado, DC Rocha, LN Santos… - Frontiers in Plant …, 2024 - frontiersin.org
Gene editing technologies have opened up the possibility of manipulating the genome of
any organism in a predicted way. CRISPR technology is the most used genome editing tool …

The type V effectors for CRISPR/Cas-mediated genome engineering in plants

R Zhang, N Chai, T Liu, Z Zheng, Q Lin, X Xie… - Biotechnology …, 2024 - Elsevier
A plethora of CRISPR effectors, such as Cas3, Cas9, and Cas12a, are commonly employed
as gene editing tools. Among these, Cas12 effectors developed based on Class II type V …