Abstract Three-dimensional (3D) cell culture has tremendous advantages to closely mimic
the in vivo architecture and microenvironment of healthy tissue and organs, as well as of …

Achieving sufficient coverage of regulatory phosphorylation sites by mass spectrometry (MS)-
based phosphoproteomics for signaling pathway reconstitution is challenging, especially …

The structure of tumors can be recapitulated as an elastic frame formed by the connected
cytoskeletons of the cells invaded by interstitial and intracellular fluids. The low-frequency …

Colorectal cancer (CRC) is a leading cause of death worldwide. Improved preclinical tumor
models are needed to make treatment screening clinically relevant and address disease …

Summary 3D in vitro cancer models are important therapeutic and biological discovery tools,
yet formation of matrix-embedded multicellular spheroids prepared in high-throughput …

Hypoxia (low oxygen levels) occurs in a range of biological contexts, including plants,
bacterial biofilms, and solid tumors; it elicits responses from these biological systems that …

Simple Summary Limited volumetric change after pre-operative radiotherapy (RT) suggests
that sarcomas generally do not undergo cell death. Senolytic drugs represent a highly …

The major underlying cause for the high mortality rate in colorectal cancer (CRC) relies on
its drug resistance, to which intratumor heterogeneity (ITH) contributes substantially. CRC …

Targeted drug delivery offers great opportunities for treating cancer. Here, we developed a
novel anticancer targeted delivery system for piperine (Pip), an alkaloid prodrug derived …

The huge gap between 2D in vitro assays used for drug screening and the in vivo 3D
physiological environment hampered reliable predictions for the route and accumulation of …