Prime editing for precise and highly versatile genome manipulation
Programmable gene-editing tools have transformed the life sciences and have shown
potential for the treatment of genetic disease. Among the CRISPR–Cas technologies that …
potential for the treatment of genetic disease. Among the CRISPR–Cas technologies that …
Current applications and future perspective of CRISPR/Cas9 gene editing in cancer
SW Wang, C Gao, YM Zheng, L Yi, JC Lu, XY Huang… - Molecular cancer, 2022 - Springer
Clustered regularly interspaced short palindromic repeats (CRISPR) system provides
adaptive immunity against plasmids and phages in prokaryotes. This system inspires the …
adaptive immunity against plasmids and phages in prokaryotes. This system inspires the …
Evolution of an adenine base editor into a small, efficient cytosine base editor with low off-target activity
Cytosine base editors (CBEs) are larger and can suffer from higher off-target activity or lower
on-target editing efficiency than current adenine base editors (ABEs). To develop a CBE that …
on-target editing efficiency than current adenine base editors (ABEs). To develop a CBE that …
Precise plant genome editing using base editors and prime editors
The development of CRISPR–Cas systems has sparked a genome editing revolution in
plant genetics and breeding. These sequence-specific RNA-guided nucleases can induce …
plant genetics and breeding. These sequence-specific RNA-guided nucleases can induce …
Efficient in vivo base editing via single adeno-associated viruses with size-optimized genomes encoding compact adenine base editors
The viral delivery of base editors has been complicated by their size and by the limited
packaging capacity of adeno-associated viruses (AAVs). Typically, dual-AAV approaches …
packaging capacity of adeno-associated viruses (AAVs). Typically, dual-AAV approaches …
Genome editing with CRISPR–Cas nucleases, base editors, transposases and prime editors
The development of new CRISPR–Cas genome editing tools continues to drive major
advances in the life sciences. Four classes of CRISPR–Cas-derived genome editing agents …
advances in the life sciences. Four classes of CRISPR–Cas-derived genome editing agents …
Re-engineering the adenine deaminase TadA-8e for efficient and specific CRISPR-based cytosine base editing
Cytosine base editors (CBEs) efficiently generate precise C· G-to-T· A base conversions, but
the activation-induced cytidine deaminase/apolipoprotein B mRNA-editing enzyme catalytic …
the activation-induced cytidine deaminase/apolipoprotein B mRNA-editing enzyme catalytic …
Engineering a precise adenine base editor with minimal bystander editing
L Chen, S Zhang, N Xue, M Hong, X Zhang… - Nature Chemical …, 2023 - nature.com
Adenine base editors (ABEs) catalyze A-to-G transitions showing broad applications, but
their bystander mutations and off-target editing effects raise safety concerns. Through …
their bystander mutations and off-target editing effects raise safety concerns. Through …
CRISPR C-to-G base editors for inducing targeted DNA transversions in human cells
CRISPR-guided DNA cytosine and adenine base editors are widely used for many
applications,,–but primarily create DNA base transitions (that is, pyrimidine-to-pyrimidine or …
applications,,–but primarily create DNA base transitions (that is, pyrimidine-to-pyrimidine or …
Base editing: advances and therapeutic opportunities
EM Porto, AC Komor, IM Slaymaker… - Nature Reviews Drug …, 2020 - nature.com
Base editing—the introduction of single-nucleotide variants (SNVs) into DNA or RNA in
living cells—is one of the most recent advances in the field of genome editing. As around …
living cells—is one of the most recent advances in the field of genome editing. As around …