Many genetic diseases and undesirable traits are due to base-pair alterations in genomic
DNA. Base-editing, the newest evolution of clustered regularly interspaced short palindromic …

Genome editing (GE) has revolutionized biological research through the new ability to
precisely edit the genomes of living organisms. In recent years, various GE tools have been …

Genome editing technologies operate by inducing site-specific DNA perturbations that are
resolved by cellular DNA repair pathways. Products of genome editors include DNA breaks …

The success of base editors for the study and treatment of genetic diseases depends on the
ability to deliver them in vivo to the relevant cell types. Delivery via adeno-associated viruses …

Cytosine and adenine base editors (CBEs and ABEs) are promising new tools for achieving
the precise genetic changes required for disease treatment and trait improvement. However …

A key limitation of the use of the CRISPR–Cas9 system for genome editing and other
applications is the requirement that a protospacer adjacent motif (PAM) be present at the …

Cytosine base editors (CBEs) enable targeted C• G-to-T• A conversions in genomic DNA.
Recent studies report that BE3, the original CBE, induces a low frequency of genome-wide …

Mutations in DNA damage response (DDR) genes endanger genome integrity and
predispose to cancer and genetic disorders. Here, using CRISPR-dependent cytosine base …

Genome editing holds promise for correcting pathogenic mutations. However, it is difficult to
determine off-target effects of editing due to single-nucleotide polymorphism in individuals …

Despite the availability of Cas9 variants with varied protospacer-adjacent motif (PAM)
compatibilities, some genomic loci—especially those with pyrimidine-rich PAM sequences …