[HTML][HTML] CRISPR-based diagnostics
MM Kaminski, OO Abudayyeh, JS Gootenberg… - Nature Biomedical …, 2021 - nature.com
The accurate and timely diagnosis of disease is a prerequisite for efficient therapeutic
intervention and epidemiological surveillance. Diagnostics based on the detection of nucleic …
intervention and epidemiological surveillance. Diagnostics based on the detection of nucleic …
The CRISPR–Cas toolbox for analytical and diagnostic assay development
Y Tang, L Gao, W Feng, C Guo, Q Yang, F Li… - Chemical Society …, 2021 - pubs.rsc.org
Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR-
associated (Cas) systems have revolutionized biological and biomedical sciences in many …
associated (Cas) systems have revolutionized biological and biomedical sciences in many …
[HTML][HTML] Multiplexed CRISPR-based microfluidic platform for clinical testing of respiratory viruses and identification of SARS-CoV-2 variants
Abstract The coronavirus disease 2019 (COVID-19) pandemic has demonstrated a clear
need for high-throughput, multiplexed and sensitive assays for detecting severe acute …
need for high-throughput, multiplexed and sensitive assays for detecting severe acute …
[HTML][HTML] A paper-based assay for the colorimetric detection of SARS-CoV-2 variants at single-nucleotide resolution
The evolution of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has
highlighted the need for versatile diagnostic assays that can discriminate among emerging …
highlighted the need for versatile diagnostic assays that can discriminate among emerging …
[HTML][HTML] Fast and sensitive detection of SARS-CoV-2 RNA using suboptimal protospacer adjacent motifs for Cas12a
S Lu, X Tong, Y Han, K Zhang, Y Zhang… - Nature biomedical …, 2022 - nature.com
CRISPR-based assays for the detection of nucleic acids are highly specific, yet they are not
fast, sensitive or easy to use. Here we report a one-step fluorescence assay for the detection …
fast, sensitive or easy to use. Here we report a one-step fluorescence assay for the detection …
[HTML][HTML] Rapid detection of SARS-CoV-2 RNA in saliva via Cas13
SS Chandrasekaran, S Agrawal, A Fanton… - Nature Biomedical …, 2022 - nature.com
Rapid nucleic acid testing is central to infectious disease surveillance. Here, we report an
assay for rapid COVID-19 testing and its implementation in a prototype microfluidic device …
assay for rapid COVID-19 testing and its implementation in a prototype microfluidic device …
[HTML][HTML] An engineered CRISPR-Cas12a variant and DNA-RNA hybrid guides enable robust and rapid COVID-19 testing
Extensive testing is essential to break the transmission of SARS-CoV-2, which causes the
ongoing COVID-19 pandemic. Here, we present a CRISPR-based diagnostic assay that is …
ongoing COVID-19 pandemic. Here, we present a CRISPR-based diagnostic assay that is …
[HTML][HTML] Programmable CRISPR-Cas9 microneedle patch for long-term capture and real-time monitoring of universal cell-free DNA
B Yang, J Kong, X Fang - Nature Communications, 2022 - nature.com
Recent advances in biointerfaces have led to the development of wearable devices that can
provide insights into personal health. As wearable modules, microneedles can extract …
provide insights into personal health. As wearable modules, microneedles can extract …
Characterization of a thermostable Cas13 enzyme for one-pot detection of SARS-CoV-2
Type VI CRISPR-Cas systems have been repurposed for various applications such as gene
knockdown, viral interference, and diagnostics. However, the identification and …
knockdown, viral interference, and diagnostics. However, the identification and …
Droplet Cas12a assay enables DNA quantification from unamplified samples at the single-molecule level
DNA quantification is important for biomedical research, but the routinely used techniques
rely on nucleic acid amplification which have inherent issues like cross-contamination risk …
rely on nucleic acid amplification which have inherent issues like cross-contamination risk …