Precise plant genome editing using base editors and prime editors
The development of CRISPR–Cas systems has sparked a genome editing revolution in
plant genetics and breeding. These sequence-specific RNA-guided nucleases can induce …
plant genetics and breeding. These sequence-specific RNA-guided nucleases can induce …
Genome editing with CRISPR–Cas nucleases, base editors, transposases and prime editors
The development of new CRISPR–Cas genome editing tools continues to drive major
advances in the life sciences. Four classes of CRISPR–Cas-derived genome editing agents …
advances in the life sciences. Four classes of CRISPR–Cas-derived genome editing agents …
Base editing: advances and therapeutic opportunities
EM Porto, AC Komor, IM Slaymaker… - Nature Reviews Drug …, 2020 - nature.com
Base editing—the introduction of single-nucleotide variants (SNVs) into DNA or RNA in
living cells—is one of the most recent advances in the field of genome editing. As around …
living cells—is one of the most recent advances in the field of genome editing. As around …
Identification of drug transporter genomic variants and inhibitors that protect against doxorubicin-induced cardiotoxicity
Background: Multiple pharmacogenomic studies have identified the synonymous genomic
variant rs7853758 (G> A, L461L) and the intronic variant rs885004 in SLC28A3 (solute …
variant rs7853758 (G> A, L461L) and the intronic variant rs885004 in SLC28A3 (solute …
[HTML][HTML] Current status and challenges of DNA base editing tools
CRISPR-mediated DNA base editors, which include cytosine base editors (CBEs) and
adenine base editors (ABEs), are promising tools that can induce point mutations at desired …
adenine base editors (ABEs), are promising tools that can induce point mutations at desired …
[HTML][HTML] Expanding the CRISPR toolbox in zebrafish for studying development and disease
The study of model organisms has revolutionized our understanding of the mechanisms
underlying normal development, adult homeostasis, and human disease. Much of what we …
underlying normal development, adult homeostasis, and human disease. Much of what we …
Next-generation CRISPR technologies and their applications in gene and cell therapy
T Gaj - Trends in biotechnology, 2021 - cell.com
The emergence of clustered regularly interspaced short palindromic repeat (CRISPR)
nucleases has transformed biotechnology by providing an easy, efficient, and versatile …
nucleases has transformed biotechnology by providing an easy, efficient, and versatile …
[HTML][HTML] PnB Designer: a web application to design prime and base editor guide RNAs for animals and plants
Background The rapid expansion of the CRISPR toolbox through tagging effector domains
to either enzymatically inactive Cas9 (dCas9) or Cas9 nickase (nCas9) has led to several …
to either enzymatically inactive Cas9 (dCas9) or Cas9 nickase (nCas9) has led to several …
Microbial base editing: a powerful emerging technology for microbial genome engineering
Genome engineering is crucial for answering fundamental questions about, and exploring
practical applications of, microorganisms. Various microbial genome-engineering tools …
practical applications of, microorganisms. Various microbial genome-engineering tools …
Design of guide RNA for CRISPR/Cas plant genome editing
GA Gerashchenkov, NA Rozhnova, BR Kuluev… - Molecular Biology, 2020 - Springer
CRISPR/Cas technology of genome editing is a powerful tool for making targeted changes
in the DNA of various organisms, including plants. The choice of the precise nucleotide …
in the DNA of various organisms, including plants. The choice of the precise nucleotide …