Although next-generation sequencing (NGS) technology revolutionized sequencing, offering
a tremendous sequencing capacity with groundbreaking depth and accuracy, it continues to …

Various next generation sequencing (NGS) based strategies have been successfully used in
the recent past for tracing origins and understanding the evolution of infectious agents …

Assessment of bacterial diversity through sequencing of 16S ribosomal RNA (16S rRNA)
genes has been an approach widely used in environmental microbiology, particularly since …

Aligning sequencing reads onto a reference is an essential step of the majority of genomic
analysis pipelines. Computational algorithms for read alignment have evolved in …

Sludge from wastewater treatment plants can act as a repository and crucial environmental
provider of antibiotic resistance genes (ARGs). Over the past few years, people's knowledge …

Eukaryotic genome sequencing and de novo assembly, once the exclusive domain of well-
funded international consortia, have become increasingly affordable, thus fitting the budgets …

For the past two decades, microbial monitoring of the International Space Station (ISS) has
relied on culture-dependent methods that require return to Earth for analysis. This has a …

In recent years, many different methods that allow for the analysis of the biodiversity and
structure of the community of microorganisms inhabiting the soil environment have emerged …

High-quality genome chromosome-scale sequences provide an important basis for
genomics downstream analysis, especially the construction of haplotype-resolved and …

The commercial release of third-generation sequencing technologies (TGSTs), giving long
and ultra-long sequencing reads, has stimulated the development of new tools for …