According to Darwin's theory, endless evolution leads to a revolution. One such example is
the Clustered Regularly Interspaced Palindromic Repeats (CRISPR)–Cas system, an …

The in-depth study of fungal secondary metabolites (SMs) over the past few years has led to
the discovery of a vast number of novel fungal SMs, some of which possess good biological …

The clustered regularly interspaced short palindromic repeats/CRISPR-associated protein
(CRISPR/Cas9) system is a powerful genome editing tool that has been successfully …

CRISPR/Cas technology is a powerful tool for genome engineering in Aspergillus oryzae as
an industrially important filamentous fungus. Previous study has reported the application of …

For Aspergillus flavus, a pathogen of considerable economic and health concern, successful
gene knockout work for more than a decade has relied nearly exclusively on using …

The traditional Japanese single distilled liquor, which uses koji and yeast with designated
ingredients, is called “honkaku shochu.” It is made using local agricultural products and has …

Kojic acid is an industrially important secondary metabolite produced by Aspergillus oryzae.
The construction of genetic materials for kojic acid related genes is important for …

Species of the genus Aspergillus have a variety of effects on humans and have been
considered industrial cell factories due to their prominent ability for manufacturing several …

Aspergillus sojae has traditionally been used in soy sauce brewing. Genetic modification
techniques have been established in A. sojae, but it is difficult to apply them to various …

Abstract CRISPR/Cas9-mediated homology-directed recombination is an efficient method to
express target genes. Based on the above method, providing ideal neutral integration sites …