Developing economically viable, scalable, and sustainable technologies for the conversion
of lignocellulosic polysaccharides to liquid fuels is widely seen as a centerpiece of the …

Infectious diseases, along with cancers, are among the main causes of death among
humans worldwide. The production of therapeutic proteins for treating diseases at large …

Existing technologies for site-specific integration of kilobase-sized DNA sequences in
bacteria are limited by low efficiency, a reliance on recombination, the need for multiple …

Genome editing in bacteria encompasses a wide array of laborious and multi-step methods
such as suicide plasmids. The discovery and applications of clustered regularly interspaced …

High levels of anthropogenic CO 2 emissions are driving the warming of global climate. If
this pattern of increasing emissions does not change, it will cause further climate change …

Studies of the gut microbiota have revealed associations between specific bacterial species
or community compositions with health and disease, yet the causal mechanisms underlying …

Non-model microorganisms often possess complex phenotypes that could be important for
the future of biofuel and chemical production. They have received significant interest the last …

With global demands for mineral resources increasing and ore grades decreasing,
microorganisms have been increasingly deployed in biomining applications to recover …

Synthetic sRNAs allow knockdown of target genes at translational level, but have been
restricted to a limited number of bacteria. Here, we report the development of a broad-host …

The genetic modification of microorganisms is conducive to the selection of high-yield
producers of high-value-added chemicals, but a lack of genetic tools hinders the …