Acoustofluidic separation of cells and particles

M Wu, A Ozcelik, J Rufo, Z Wang, R Fang… - Microsystems & …, 2019 - nature.com
Acoustofluidics, the integration of acoustics and microfluidics, is a rapidly growing research
field that is addressing challenges in biology, medicine, chemistry, engineering, and …

Contributions to the dynamics of cervix remodeling prior to term and preterm birth

SM Yellon - Biology of reproduction, 2017 - academic.oup.com
Major clinical challenges for obstetricians and neonatologists result from early cervix
remodeling and preterm birth. Complications related to cervix remodeling or delivery …

Amine reactive dyes: an effective tool to discriminate live and dead cells in polychromatic flow cytometry

SP Perfetto, PK Chattopadhyay, L Lamoreaux… - Journal of …, 2006 - Elsevier
Membrane-damaged cells caused by either mechanical trauma or through normal biological
processes can produce artifacts in immunophenotyping analysis by flow cytometry. Dead …

The histone deacetylase inhibitor ITF2357 selectively targets cells bearing mutated JAK2V617F

V Guerini, V Barbui, O Spinelli, A Salvi, C Dellacasa… - Leukemia, 2008 - nature.com
We investigated the activity of ITF2357, a novel histone deacetylase inhibitor (HDACi) with
antitumor activity, on cells carrying the JAK2 V617F mutation obtained from polycythemia …

Amine‐reactive dyes for dead cell discrimination in fixed samples

SP Perfetto, PK Chattopadhyay… - Current protocols in …, 2010 - Wiley Online Library
Amine‐reactive dyes, also known as LIVE/DEAD fixable dead cell stains, are a class of
viability dyes suitable for identifying dead cells in samples that will be fixed. These dyes …

Detection of dead cells and measurement of cell killing by flow cytometry

MA King - Journal of immunological methods, 2000 - Elsevier
A flow cytometer can quickly perform numerous quantitative, sensitive measurements on
each individual cell within a large, heterogeneous population. The modern commercially …

Live-cell assay for detection of apoptosis by dual-laser flow cytometry using Hoechst 33342 and 7-amino-actinomycin D

I Schmid, C Uittenbogaart, BD Jamieson - Nature Protocols, 2007 - nature.com
This protocol describes a rapid and simple method for the identification of apoptotic cells.
Owing to changes in membrane permeability, early apoptotic cells show an increased …

Measuring DNA content in live cells by fluorescence microscopy

CJ Gomes, MW Harman, SM Centuori, CW Wolgemuth… - Cell Division, 2018 - Springer
Background Live-cell fluorescence microscopy (LCFM) is a powerful tool used to investigate
cellular dynamics in real time. However, the capacity to simultaneously measure DNA …

Early Apoptosis Largely Accounts for Functional Impairment of CD34+ Cells in Frozen-Thawed Stem Cell Grafts

F De Boer, AM Dräger, HM Pinedo… - … of hematotherapy & …, 2002 - liebertpub.com
Quality assessment of stem cell grafts is usually performed by flow cytometric CD34+
enumeration or assessment of clonogenic output of fresh material. Previously, we identified …

Extensive early apoptosis in frozen–thawed CD34-positive stem cells decreases threshold doses for haematological recovery after autologous peripheral blood …

F De Boer, AM Dräger, HM Pinedo, FL Kessler… - Bone marrow …, 2002 - nature.com
Stem cell doses necessary for engraftment after myelo-ablative therapy as defined for fresh
transplants vary largely. Loss of CD34+ cell quality after cryopreservation might contribute to …