Fluorescence lifetime imaging microscopy: fundamentals and advances in instrumentation, analysis, and applications

R Datta, TM Heaster, JT Sharick… - … of biomedical optics, 2020 - spiedigitallibrary.org
Significance: Fluorescence lifetime imaging microscopy (FLIM) is a powerful technique to
distinguish the unique molecular environment of fluorophores. FLIM measures the time a …

Phosphorescent iridium (III) complexes as lifetime-based biological sensors for photoluminescence lifetime imaging microscopy

J Zhou, J Li, KY Zhang, S Liu, Q Zhao - Coordination Chemistry Reviews, 2022 - Elsevier
Luminescent iridium (III) complexes exhibit high phosphorescence quantum yields and
photostability, good cell membrane permeability, rich and sensitive photophysical …

Label-free sensing of cells with fluorescence lifetime imaging: the quest for metabolic heterogeneity

EA Shirshin, MV Shirmanova… - Proceedings of the …, 2022 - National Acad Sciences
Molecular, morphological, and physiological heterogeneity is the inherent property of cells
which governs differences in their response to external influence. Tumor cell metabolic …

Recent progress in functionalized and targeted polymersomes and chimeric polymeric nanotheranostic platforms for cancer therapy

M Beygi, F Oroojalian, SS Hosseini… - Progress in Materials …, 2023 - Elsevier
The majority of advances in cancer therapy have been achieved based on developments in
public awareness, as well as novel diagnostic and therapeutic modalities. Treatment …

[HTML][HTML] Compressed sensing in fluorescence microscopy

G Calisesi, A Ghezzi, D Ancora, C D'Andrea… - Progress in Biophysics …, 2022 - Elsevier
Compressed sensing (CS) is a signal processing approach that solves ill-posed inverse
problems, from under-sampled data with respect to the Nyquist criterium. CS exploits …

FLIM as a promising tool for cancer diagnosis and treatment monitoring

Y Ouyang, Y Liu, ZM Wang, Z Liu, M Wu - Nano-Micro Letters, 2021 - Springer
Fluorescence lifetime imaging microscopy (FLIM) has been rapidly developed over the past
30 years and widely applied in biomedical engineering. Recent progress in fluorophore …

Current advances in lipid nanosystems intended for topical and transdermal drug delivery applications

N Akombaetwa, AB Ilangala, L Thom, PB Memvanga… - Pharmaceutics, 2023 - mdpi.com
Skin delivery is an exciting and challenging field. It is a promising approach for effective drug
delivery due to its ease of administration, ease of handling, high flexibility, controlled …

Molecularly Engineered Room-Temperature Phosphorescence for Biomedical Application: From the Visible toward Second Near-Infrared Window

B Chang, J Chen, J Bao, T Sun, Z Cheng - Chemical Reviews, 2023 - ACS Publications
Phosphorescence, characterized by luminescent lifetimes significantly longer than that of
biological autofluorescence under ambient environment, is of great value for biomedical …

Short‐wave Infrared Photoluminescence Lifetime Mapping of Rare‐Earth Doped Nanoparticles Using All‐Optical Streak Imaging

M Liu, Y Lai, M Marquez, F Vetrone… - Advanced Science, 2024 - Wiley Online Library
The short‐wave infrared (SWIR) photoluminescence lifetimes of rare‐earth doped
nanoparticles (RENPs) have found diverse applications in fundamental and applied …

Fluorescence lifetime imaging with a megapixel SPAD camera and neural network lifetime estimation

V Zickus, ML Wu, K Morimoto, V Kapitany, A Fatima… - Scientific Reports, 2020 - nature.com
Fluorescence lifetime imaging microscopy (FLIM) is a key technology that provides direct
insight into cell metabolism, cell dynamics and protein activity. However, determining the …