The ease of genetic manipulation, low cost, rapid growth and number of previous studies
have made Escherichia coli one of the most widely used microorganism species for …

Proteins are now widely produced in diverse microbial cell factories. The Escherichia coli is
still the dominant host for recombinant protein production but, as a bacterial cell, it also has …

Ubiquitin-binding proteins play an important role in eukaryotes by translating differently
linked polyubiquitin chains into proper cellular responses. Current knowledge about …

It is important to assess the identity and purity of proteins and protein complexes during and
after protein purification to ensure that samples are of sufficient quality for further …

In the late 70's, the discovery of the restriction enzymes made possible the biological
production of functional proteins by recombinant DNA technologies, a fact that largely …

Peptide tags are a key resource, introducing minimal change while enabling a consistent
process to purify diverse proteins. However, peptide tags often provide minimal benefit post …

Metals have crucial roles in many physiological, pathological, toxicological, pharmaceutical,
and diagnostic processes. Proper handling of metal-containing macromolecule samples for …

Fusion expression is a common practice for recombinant protein production. Some fusion
tags confer solubility on the target protein whereas others provide affinity handles that …

The lack of cost-effective methods for producing antimicrobial peptides has made it
impossible to use their high potential as a new and powerful class of antimicrobial agents. In …

Advancements in peptide fusion technologies to maximize the protein production has taken
a big leap to fulfill the demands of post-genomics era targeting elucidation of …