Challenges and opportunities in exosome research—Perspectives from biology, engineering, and cancer therapy
ABSTRACT Exosomes are small ($30–140 nm) lipid bilayer-enclosed particles of
endosomal origin. They are a subset of extracellular vesicles (EVs) that are secreted by most …
endosomal origin. They are a subset of extracellular vesicles (EVs) that are secreted by most …
Protein purification and analysis: next generation Western blotting techniques
Introduction: Western blotting is one of the most commonly used techniques in molecular
biology and proteomics. Since western blotting is a multistep protocol, variations and errors …
biology and proteomics. Since western blotting is a multistep protocol, variations and errors …
[HTML][HTML] Correlation of mRNA and protein in complex biological samples
The correlation between mRNA and protein abundances in the cell has been reported to be
notoriously poor. Recent technological advances in the quantitative analysis of mRNA and …
notoriously poor. Recent technological advances in the quantitative analysis of mRNA and …
Proteomic challenges: sample preparation techniques for microgram-quantity protein analysis from biological samples
P Feist, AB Hummon - International journal of molecular sciences, 2015 - mdpi.com
Proteins regulate many cellular functions and analyzing the presence and abundance of
proteins in biological samples are central focuses in proteomics. The discovery and …
proteins in biological samples are central focuses in proteomics. The discovery and …
Proteome turnover in the spotlight: approaches, applications, and perspectives
In all cells, proteins are continuously synthesized and degraded to maintain protein
homeostasis and modify gene expression levels in response to stimuli. Collectively, the …
homeostasis and modify gene expression levels in response to stimuli. Collectively, the …
Electrochemistry of nonconjugated proteins and glycoproteins. Toward sensors for biomedicine and glycomics
The present advances in biology are related to progress in genomics, proteomics, and other
“-omics”, including glycomics, working with a large amount of data regarding human and …
“-omics”, including glycomics, working with a large amount of data regarding human and …
A review on the enzymes and metabolites identified by mass spectrometry from bacteria and microalgae involved in the degradation of high molecular weight PAHs
MM García, MPG de Llasera - Science of the Total Environment, 2021 - Elsevier
High molecular weight PAHs (HMW PAHs) are dangerous pollutants widely distributed in
the environment. The use of microorganisms represents an important tool for HMW PAHs …
the environment. The use of microorganisms represents an important tool for HMW PAHs …
[HTML][HTML] Proteomics: challenges, techniques and possibilities to overcome biological sample complexity
K Chandramouli, PY Qian - Human genomics and proteomics: HGP, 2009 - ncbi.nlm.nih.gov
Proteomics is the large-scale study of the structure and function of proteins in complex
biological sample. Such an approach has the potential value to understand the complex …
biological sample. Such an approach has the potential value to understand the complex …
Follicular fluid content and oocyte quality: from single biochemical markers to metabolomics
A Revelli, LD Piane, S Casano, E Molinari… - Reproductive biology …, 2009 - Springer
The assessment of oocyte quality in human in vitro fertilization (IVF) is getting increasing
attention from embryologists. Oocyte selection and the identification of the best oocytes, in …
attention from embryologists. Oocyte selection and the identification of the best oocytes, in …