Immunoglobulin G (IgG) glycosylation critically modulates antibody effector functions.
Streptococcus pyogenes secretes a unique endo-β-N-acetylglucosaminidase, EndoS2 …

N-glycosylation on IgG modulates Fc conformation and effector functions. An IgG-Fc
contains a human sialo-complex type (hSCT) glycan of biantennary structure with two α2, 6 …

Quantifying the glycan expression status on cell surfaces is of vital importance for insight into
the glycan function in biological processes and related diseases. Here we developed a …

Fujita M., et al. A novel disaccharide Substrate having 1, 2-oxazoline moiety for detection of
transglycosylating activity of endoglycosidases. Biochim. Biophys. Acta, 2001, 1528, 9-14 …

The advent of monoclonal antibody biosimilar products has stimulated the development of
analytical methods that can better characterize an important quality attribute, namely the …

Monoclonal antibodies (mAbs) comprise an essential type of biologic therapeutics and are
used to treat diseases because of their anti-cancer and anti-inflammatory properties, and …

Advances in genetic and protein engineering and the ability to maintain proliferating
mammalian cells in vitro, has allowed reverse engineering of antibodies, ie generation of …

Generally speaking, nuclear magnetic resonance (NMR) spectroscopy seems the most
powerful technique in current use for structural analysis of biomolecules. Four Nobel prizes …

In spite of the abundance of glycoproteins in biological processes, relatively little three-
dimensional structural data is available for glycan structures. Here, we study the structure …

Glycosylation is one of the most frequent post-translational modification of proteins. Many
membrane and secreted proteins are decorated by sugar chains mainly linked to …