A user's guide to golden gate cloning methods and standards
JE Bird, J Marles-Wright, A Giachino - ACS Synthetic Biology, 2022 - ACS Publications
The continual demand for specialized molecular cloning techniques that suit a broad range
of applications has driven the development of many different cloning strategies. One method …
of applications has driven the development of many different cloning strategies. One method …
[HTML][HTML] A navigation guide of synthetic biology tools for Pseudomonas putida
Pseudomonas putida is a microbial chassis of huge potential for industrial and
environmental biotechnology, owing to its remarkable metabolic versatility and ability to …
environmental biotechnology, owing to its remarkable metabolic versatility and ability to …
SEVA 4.0: an update of the Standard European Vector Architecture database for advanced analysis and programming of bacterial phenotypes
E Martínez-García, S Fraile, E Algar… - Nucleic Acids …, 2023 - academic.oup.com
Abstract The SEVA platform (https://seva-plasmids. com) was launched one decade ago,
both as a database (DB) and as a physical repository of plasmid vectors for genetic analysis …
both as a database (DB) and as a physical repository of plasmid vectors for genetic analysis …
Enabling one-pot Golden Gate assemblies of unprecedented complexity using data-optimized assembly design
DNA assembly is an integral part of modern synthetic biology, as intricate genetic
engineering projects require robust molecular cloning workflows. Golden Gate assembly is a …
engineering projects require robust molecular cloning workflows. Golden Gate assembly is a …
[HTML][HTML] Paving the way for synthetic C1-metabolism in Pseudomonas putida through the reductive glycine pathway
Abstract One-carbon (C1) compounds such as methanol, formate, and CO 2 are alternative,
sustainable microbial feedstocks for the biobased production of chemicals and fuels. In this …
sustainable microbial feedstocks for the biobased production of chemicals and fuels. In this …
Portable bacterial CRISPR transcriptional activation enables metabolic engineering in Pseudomonas putida
CRISPR-Cas transcriptional programming in bacteria is an emerging tool to regulate gene
expression for metabolic pathway engineering. Here we implement CRISPR-Cas …
expression for metabolic pathway engineering. Here we implement CRISPR-Cas …
Metagenomics harvested genus-specific single-stranded DNA-annealing proteins improve and expand recombineering in Pseudomonas species
E Asin-Garcia, L Garcia-Morales… - Nucleic acids …, 2023 - academic.oup.com
The widespread Pseudomonas genus comprises a collection of related species with
remarkable abilities to degrade plastics and polluted wastes and to produce a broad set of …
remarkable abilities to degrade plastics and polluted wastes and to produce a broad set of …
Research progress of engineering microbial cell factories for pigment production
S Xu, S Gao, Y An - Biotechnology Advances, 2023 - Elsevier
Pigments are widely used in people's daily life, such as food additives, cosmetics,
pharmaceuticals, textiles, etc. In recent years, the natural pigments produced by …
pharmaceuticals, textiles, etc. In recent years, the natural pigments produced by …
An update of the unceasingly growing and diverse AraC/XylS family of transcriptional activators
D Cortés-Avalos, N Martínez-Pérez… - FEMS Microbiology …, 2021 - academic.oup.com
Transcriptional factors play an important role in gene regulation in all organisms, especially
in Bacteria. Here special emphasis is placed in the AraC/XylS family of transcriptional …
in Bacteria. Here special emphasis is placed in the AraC/XylS family of transcriptional …
High‐Complexity One‐Pot Golden Gate Assembly
Abstract Golden Gate Assembly is a flexible method of DNA assembly and cloning that
permits the joining of multiple fragments in a single reaction through predefined …
permits the joining of multiple fragments in a single reaction through predefined …