The worldwide prevalence of diabetes mellitus and obesity is rapidly increasing not only in
adults but also in children and adolescents. Diabetes is associated with macrovascular …

Huntington's disease (HD) is characterized by preferential loss of the medium spiny neurons
in the striatum. Using CRISPR/Cas9 and somatic nuclear transfer technology, we …

This review will detail the current status of islet xenotransplantation as a potential treatment
for type I diabetes mellitus, focusing on recent advances in porcine xenogeneic islet …

Cytosine base editors (CBEs) enable programmable C-to-T conversion without DNA double-
stranded breaks and homology-directed repair in a variety of organisms, which exhibit great …

Insulin-like growth factor 2 (IGF2) is an important growth factor, which promotes growth and
development in mammals during fetal and postnatal stages. Using CRISPR–Cas9 system …

Despite being time-consuming and costly, generating genome-edited pigs holds great
promise for agricultural, biomedical, and pharmaceutical applications. To further facilitate …

Islet allotransplantation results in increasing success in treating type 1 diabetes, but the
shortage of deceased human donor pancreata limits progress. Islet xenotransplantation …

Genetically modified animals, especially rodents, are widely used in biomedical research.
However, non-rodent models are required for efficient translational medicine and preclinical …

Monogenic disorders (MGDs), which are caused by single gene mutations, have a serious
effect on human health. Among these, β-thalassemia (β-thal) represents one of the most …

Since its emergence, the clustered regularly interspaced short palindromic repeat (CRISPR)–
CRISPR-associated (Cas) 9 system has been increasingly used to generate animals for …