According to Darwin's theory, endless evolution leads to a revolution. One such example is
the Clustered Regularly Interspaced Palindromic Repeats (CRISPR)–Cas system, an …

Production of industrial chemicals using renewable biomass feedstock is becoming
increasingly important to address limited fossil resources, climate change and other …

As important genome editing tools, CRISPR/Cas systems, especially those based on type II
Cas9 and type V Cas12a, are widely used in genetic and metabolic engineering of bacteria …

Lignin is one of the most abundant renewable resources on earth and is readily produced as
a sidestream during biomass fractioning. So far, these large quantities of lignin have been …

Amino acid fermentation is one of the major pillars of industrial biotechnology. The multi-
billion USD amino acid market is rising steadily and is diversifying. Metabolic engineering is …

Corynebacterium glutamicum is a major workhorse in industrial biotechnology. For the past
several decades, this soil bacterium has been used to produce the amino acids l-glutamate …

Metabolic engineering allows development of microbial strains efficiently producing
chemicals and materials, but it requires much time, effort, and cost to make the strains …

Abstract Development of hyperproducing strains is important for biomanufacturing of
biochemicals and biofuels but requires extensive efforts to engineer cellular metabolism and …

CRISPR base editing is a powerful method to engineer bacterial genomes. However, it
restricts editing to single-nucleotide substitutions. Here, to address this challenge, we adapt …

The increasing demand for the development of sustainable strategies to utilize and process
agro-industrial residues paves new paths for exploring innovative approaches in this area …