Extraction-free, one-pot CRISPR/Cas12a detection of microRNAs directly from extracellular vesicles

H Yan, S Han, S Hughes, Y Zeng - Chemical Communications, 2023 - pubs.rsc.org
Current methods for extracellular vesicle (EV) miRNA analysis mostly require RNA
extraction, which results in a multi-step, time-consuming workflow. This study reports an …

lncRNA TRPM2‐AS Promotes Colorectal Cancer Progression by Regulating miR‐22‐3p and FSTL1

Y Gao, B Liu, X Liu, Y Gao, J Shan, Y Li - Journal of Oncology, 2022 - Wiley Online Library
Background. In recent years, long noncoding RNAs (lncRNAs) relate to many biological
processes, which affect the progression of tumors. Transient receptor potential melastatin 2 …

[HTML][HTML] Emerging biomarkers for non-invasive diagnosis and treatment of cancer: a systematic review

S Zakari, NK Niels, GV Olagunju, PC Nnaji… - Frontiers in …, 2024 - ncbi.nlm.nih.gov
Cancer remains a global health challenge, necessitating continuous advancements in
diagnostic and treatment strategies. This review focuses on the utility of non-invasive …

[HTML][HTML] Regulatory role of RNA modifications in the treatment of pancreatic ductal adenocarcinoma (PDAC)

H Chen, W Luo, X Lu, T Zhang - Heliyon, 2023 - cell.com
Pancreatic ductal adenocarcinoma (PDAC) is an extremely life-threatening malignancy with
a relatively unfavorable prognosis. The early occurrence of metastasis and local recurrence …

[HTML][HTML] RT-qPCR Expression Profiles of selected oncogenic and oncosuppressor miRNAs in formalin-fixed, paraffin-embedded Canine Mammary Tumors

JM Abbate, A Giannetto, F Arfuso, B Brunetti, G Lanteri - Animals, 2022 - mdpi.com
Simple Summary Early diagnosis of mammary gland cancer would allow intervention in the
early stage of the disease, with a better prognosis for canine patients. MiRNAs are short non …

A label-free and rapid fluorometric strategy for microRNA detection using CRISPR-Cas12a coupled with copper nanoparticles

S Wang, Z Wei, L Li, Y Luo, Z Huang, X Yang, Y Tang - Microchimica Acta, 2024 - Springer
CRISPR-Cas12a with robust trans-cleavage activity were employed to mitigate background
fluorescence signal, achieving sensitive detection of miRNA-21. The activation of trans …

lncRNA NEAT1/miR‐495‐3p regulates angiogenesis in burn sepsis through the TGF‐β1 and SMAD signaling pathways

Y Meng, Z Hao, H Zhang, P Bai, W Guo… - Immunity …, 2023 - Wiley Online Library
Introduction To investigate the role of the long‐chain noncoding RNA (lncRNA) nuclear
enriched abundant transcript 1 (NEAT1) in the process of angiogenesis in human umbilical …

Bio-diagnostic performances of microRNAs set related to DNA damage response pathway among hepatitis C virus-associated hepatocellular carcinoma patients

SM Abdo, WG Shousha, AA Mohamed… - Journal of Genetic …, 2023 - Elsevier
Background Up to date, a well-defined microRNAs (miRNAs) profile involved in
hepatocellular carcinoma (HCC) pathogenesis remains indecisive. Thus, employing …

Ensemble classifiers for multiclass microRNA classification

L Odenthal, J Allmer, M Yousef - miRNomics: MicroRNA Biology and …, 2022 - Springer
Gene regulation is of utmost importance to cell homeostasis; thus, any dysregulation in it
often leads to disease. MicroRNAs (miRNAs) are involved in posttranscriptional gene …

The roles of miR-383 as a diagnostic and prognostic biomarker in human cancers

R Panahizadeh, M Vatankhah, P Gholizadeh… - Gene Reports, 2024 - Elsevier
Emerging evidence indicates that Long non-coding RNAs (LncRNAs) and microRNAs
(miRNAs) play crucial roles in the biology of cancerous cells. MicroRNAs are small non …