[HTML][HTML] Recent advances in biocatalysts engineering for polyethylene terephthalate plastic waste green recycling

NA Samak, Y Jia, MM Sharshar, T Mu, M Yang… - Environment …, 2020 - Elsevier
The massive waste of poly (ethylene terephthalate)(PET) that ends up in the landfills and
oceans and needs hundreds of years for degradation has attracted global concern. The poor …

[HTML][HTML] Microbial enzymes: tools for biotechnological processes

JL Adrio, AL Demain - Biomolecules, 2014 - mdpi.com
Microbial enzymes are of great importance in the development of industrial bioprocesses.
Current applications are focused on many different markets including pulp and paper …

Laboratory evolution of stereoselective enzymes: a prolific source of catalysts for asymmetric reactions

MT Reetz - Angewandte Chemie International Edition, 2011 - Wiley Online Library
Asymmetric catalysis plays a key role in modern synthetic organic chemistry, with synthetic
catalysts and enzymes being the two available options. During the latter part of the last …

Semi-rational approaches to engineering enzyme activity: combining the benefits of directed evolution and rational design

RA Chica, N Doucet, JN Pelletier - Current opinion in biotechnology, 2005 - Elsevier
Many research groups successfully rely on whole-gene random mutagenesis and
recombination approaches for the directed evolution of enzymes. Recent advances in …

Recombinant protein secretion in Escherichia coli

FJM Mergulhão, DK Summers, GA Monteiro - Biotechnology advances, 2005 - Elsevier
The secretory production of recombinant proteins by the Gram-negative bacterium
Escherichia coli has several advantages over intracellular production as inclusion bodies. In …

Improved biocatalysts by directed evolution and rational protein design

UT Bornscheuer, M Pohl - Current opinion in chemical biology, 2001 - Elsevier
The efficient application of biocatalysts requires the availability of suitable enzymes with
high activity and stability under process conditions, desired substrate selectivity and high …

Bacteriophage endolysins as a novel class of antibacterial agents

J Borysowski, B Weber-Dąbrowska… - … biology and medicine, 2006 - journals.sagepub.com
Endolysins are double-stranded DNA bacteriophage-encoded peptidoglycan hydrolases
produced in phage-infected bacterial cells toward the end of the lytic cycle. They reach the …

Improving enzyme properties: when are closer mutations better?

KL Morley, RJ Kazlauskas - Trends in biotechnology, 2005 - cell.com
Study of mutations that improve enzyme properties reveals that in many, but not all, cases
closer mutations are more effective than distant ones. For enantioselectivity, substrate …

Combinatorial and computational challenges for biocatalyst design

FH Arnold - Nature, 2001 - nature.com
Nature provides a fantastic array of catalysts extremely well suited to supporting life, but
usually not so well suited for technology. Whether biocatalysis will have a significant …

Parameters in preparation and characterization of cross linked enzyme aggregates (CLEAs)

S Talekar, A Joshi, G Joshi, P Kamat, R Haripurkar… - Rsc Advances, 2013 - pubs.rsc.org
In the past couple of decades, cross linked enzyme aggregates (CLEAs) have emerged as a
novel and versatile carrier free immobilization technique. The immobilization of enzymes as …