Protein semisynthesis—defined herein as the assembly of a protein from a combination of
synthetic and recombinant fragments—is a burgeoning field of chemical biology that has …

The regulation of transcription by RNA polymerase II is closely intertwined with the
regulation of chromatin structure. A host of proteins required for the disassembly …

1. INTRODUCTION In eukaryotic cells, inheritable information is stored in a nucleoprotein
complex referred to as chromatin. 1 This genome architecture serves two key purposes. On …

Whole-genome sequencing data mining efforts have revealed numerous histone mutations
in a wide range of cancer types. These occur in all four core histones in both the tail and …

Staphylococcus aureus sortase A catalyzes the transpeptidation of an LPXTG peptide
acceptor and a glycine-linked peptide donor and has proven to be a powerful tool for site …

Elucidating the molecular details of how chromatin-associated factors deposit, remove and
recognize histone post-translational modification (PTM) signatures remains a daunting task …

Monoubiquitylation of histone H2B (H2Bub1), catalyzed by the heterodimeric ubiquitin
ligase complex RNF20/40, regulates multiple molecular and biological processes. The …

Histone ubiquitination affects the structure and function of nucleosomes through tightly
regulated dynamic reversible processes. The efficient preparation of ubiquitinated histones …

Epigenetic regulators have a crucial effect on gene expression based on their manipulation
of histone modifications. Histone H2AK119 monoubiquitination (H2AK119Ub), a well …

Ubiquitination has traditionally been viewed in the context of polyubiquitination that is
essential for marking proteins for degradation via the proteasome. Recent discoveries have …