CRISPR/Cas systems in genome editing: methodologies and tools for sgRNA design, off‐target evaluation, and strategies to mitigate off‐target effects
H Manghwar, B Li, X Ding, A Hussain… - Advanced …, 2020 - Wiley Online Library
Life sciences have been revolutionized by genome editing (GE) tools, including zinc finger
nucleases, transcription activator‐Like effector nucleases, and CRISPR (clustered regulatory …
nucleases, transcription activator‐Like effector nucleases, and CRISPR (clustered regulatory …
Bio-based nanomaterials for cancer therapy
Bio-based nanomaterials have received considerable attention in cancer therapies due to
their potent characteristics such as autologous pharmaceutical, synergetic effects …
their potent characteristics such as autologous pharmaceutical, synergetic effects …
Multiplex CRISPR/Cas9‐mediated metabolic engineering increases soya bean isoflavone content and resistance to soya bean mosaic virus
P Zhang, H Du, J Wang, Y Pu, C Yang… - Plant biotechnology …, 2020 - Wiley Online Library
Isoflavonoids, which include a variety of secondary metabolites, are derived from the
phenylpropanoid pathway and are distributed predominantly in leguminous plants. These …
phenylpropanoid pathway and are distributed predominantly in leguminous plants. These …
In-Vivo Induced CAR-T Cell for the Potential Breakthrough to Overcome the Barriers of Current CAR-T Cell Therapy
T Xin, L Cheng, C Zhou, Y Zhao, Z Hu, X Wu - Frontiers in oncology, 2022 - frontiersin.org
Chimeric antigen receptor T cell (CAR-T cell) therapy has shown impressive success in the
treatment of hematological malignancies, but the systemic toxicity and complex …
treatment of hematological malignancies, but the systemic toxicity and complex …
Gene therapy to enhance angiogenesis in chronic wounds
Skin injuries and chronic non-healing wounds are one of the major global burdens on the
healthcare systems worldwide due to their difficult-to-treat nature, associated co-morbidities …
healthcare systems worldwide due to their difficult-to-treat nature, associated co-morbidities …
Evaluating and enhancing target specificity of gene-editing nucleases and deaminases
Programmable nucleases and deaminases, which include zinc-finger nucleases,
transcription activator-like effector nucleases, CRISPR RNA-guided nucleases, and RNA …
transcription activator-like effector nucleases, CRISPR RNA-guided nucleases, and RNA …
Engineered probiotics
J Ma, Y Lyu, X Liu, X Jia, F Cui, X Wu, S Deng… - Microbial cell …, 2022 - Springer
Engineered probiotics are a kind of new microorganisms produced by modifying original
probiotics through gene editing. With the continuous development of tools and technology …
probiotics through gene editing. With the continuous development of tools and technology …
Genetic etiology and clinical challenges of phenylketonuria
NA Elhawary, IA AlJahdali, IS Abumansour… - Human genomics, 2022 - Springer
This review discusses the epidemiology, pathophysiology, genetic etiology, and
management of phenylketonuria (PKU). PKU, an autosomal recessive disease, is an inborn …
management of phenylketonuria (PKU). PKU, an autosomal recessive disease, is an inborn …
Integration of CRISPR/Cas9 with artificial intelligence for improved cancer therapeutics
Gene editing has great potential in treating diseases caused by well-characterized
molecular alterations. The introduction of clustered regularly interspaced short palindromic …
molecular alterations. The introduction of clustered regularly interspaced short palindromic …
Understanding molecular plant–nematode interactions to develop alternative approaches for nematode control
MMM Abd-Elgawad - Plants, 2022 - mdpi.com
Developing control measures of plant-parasitic nematodes (PPNs) rank high as they cause
big crop losses globally. The growing awareness of numerous unsafe chemical nematicides …
big crop losses globally. The growing awareness of numerous unsafe chemical nematicides …