Main conclusion In this review, we have focused on the CRISPR/Cas9 technology for
improving the agronomic traits in plants through point mutations, knockout, and single base …

Simple Summary The fusion between sperm and oocyte results in a zygote, which is a single
totipotent cell with the ability to develop into a functional organism. Totipotent cells can give …

Societal Impact Statement A diverse portfolio of genetically engineered food crops, as well
as animal livestock and fish, are currently being developed and commercialized. To ensure …

The domestic buffalo (Bubalus bubalis), also known as water buffalo, comprises two sub-
species the River buffalo (B. bubalis ssp. bubalis; 50 chromosomes) and the Swamp buffalo …

In nonruminants, microRNA (miRNA)-24 plays an important role in lipid metabolism in
adipose tissue and the liver. Although the abundance of miR-24 in ruminant mammary …

The ability to create genetically engineered mouse models (GEMMs) has exponentially
increased our understanding of many areas of biology. Musculoskeletal biology is no …

Due to recent innovations in gene editing technology, great progress has been made in
livestock breeding, with researchers rearing gene-edited pigs, cattle, sheep, and other …

Inosine monophosphate (IMP) is an important indicator of meat freshness and contributes to
its umami taste. An attractive strategy for enhancing umami is to suppress the IMP-degrading …

The generation of mature gametes and competent embryos in vitro from pluripotent stem
cells has been successfully achieved in a few species, mainly in mice, with recent advances …

The rapid development of biotechnology has facilitated our understanding of the biological
functions of candidate genes for important economic traits in farm animals. Molecular …