[HTML][HTML] The industrial versatility of Gluconobacter oxydans: current applications and future perspectives

GAR da Silva, SSS Oliveira, SF Lima… - World Journal of …, 2022 - Springer
Gluconobacter oxydans is a well-known acetic acid bacterium that has long been applied in
the biotechnological industry. Its extraordinary capacity to oxidize a variety of sugars …

New perspectives into Gluconobacter-catalysed biotransformations

M Ripoll, JA Lerma-Escalera… - Biotechnology …, 2023 - Elsevier
Different from other aerobic microorganisms that oxidise carbon sources to water and
carbon dioxide, Gluconobacter catalyses the incomplete oxidation of various substrates with …

[HTML][HTML] Generation of a Gluconobacter oxydans knockout collection for improved extraction of rare earth elements

AM Schmitz, B Pian, S Medin, MC Reid, M Wu… - Nature …, 2021 - nature.com
Bioleaching of rare earth elements (REEs), using microorganisms such as Gluconobacter
oxydans, offers a sustainable alternative to environmentally harmful thermochemical …

A type IF CRISPRi system unveils the novel role of CzcR in modulating multidrug resistance of Pseudomonas aeruginosa

S Chen, H Cao, Z Xu, J Huang, Z Liu, T Li… - Microbiology …, 2023 - Am Soc Microbiol
Pseudomonas aeruginosa has abundant signaling systems that exquisitely control its
antibiotic resistance in response to different environmental cues. Understanding the …

Spacer2PAM: A computational framework to guide experimental determination of functional CRISPR-Cas system PAM sequences

GA Rybnicky, NA Fackler, AS Karim… - Nucleic acids …, 2022 - academic.oup.com
RNA-guided nucleases from CRISPR-Cas systems expand opportunities for precise,
targeted genome modification. Endogenous CRISPR-Cas systems in many prokaryotes are …

[HTML][HTML] CRISPR-based approaches for gene regulation in non-model bacteria

SN Call, LB Andrews - Frontiers in genome editing, 2022 - frontiersin.org
CRISPR interference (CRISPRi) and CRISPR activation (CRISPRa) have become
ubiquitous approaches to control gene expression in bacteria due to their simple design and …

[HTML][HTML] Identification of key genes through the constructed CRISPR-dcas9 to facilitate the efficient production of O-acetylhomoserine in Corynebacterium glutamicum

N Li, X Shan, J Zhou, S Yu - Frontiers in Bioengineering and …, 2022 - frontiersin.org
O-Acetylhomoserine (OAH) is an important platform chemical for the synthesis of L-
methamidophos and L-methionine. It has been produced efficiently in Corynebacterium …

[HTML][HTML] The application of CRISPR/Cas mediated gene editing in synthetic biology: Challenges and optimizations

W Li, C Huang, J Chen - Frontiers in Bioengineering and …, 2022 - frontiersin.org
Clustered regularly interspaced short palindromic repeats (CRISPR) and its associated
enzymes (Cas) is a simple and convenient genome editing tool that has been used in …

A SacB-based system for diverse and multiple genome editing in Gluconobacter oxydans

Z Qin, S Yu, L Liu, L Wang, J Chen, J Zhou - Journal of Biotechnology, 2021 - Elsevier
Gluconobacter oxydans is an important industrial bacterial strain widely used to produce a
lot of useful products. However, very few gene editing tools are available for G. oxydans …

Endogenous CRISPR-Cas mediated in situ genome editing: State-of-the-art and the road ahead for engineering prokaryotes

Z Liu, J Liu, Z Yang, L Zhu, Z Zhu, H Huang… - Biotechnology …, 2023 - Elsevier
The CRISPR-Cas systems have shown tremendous promise as heterologous tools for
genome editing in various prokaryotes. However, the perturbation of DNA homeostasis and …