CRISPR Tools in Bacterial Whole-Cell Biocatalysis

AP Mulet, M Ripoll, L Betancor - ACS Sustainable Chemistry & …, 2023 - ACS Publications
Biocatalysis has emerged as a promising alternative to conventional chemical processes for
the production of a wide range of chemicals, providing a sustainable solution to the problem …

Osmotic stress tolerance and transcriptome analysis of Gluconobacter oxydans to extra-high titers of glucose

X Liu, Z Wang, J Xiao, X Zhou, Y Xu - Frontiers in Microbiology, 2022 - frontiersin.org
Gluconobacter oxydans has been widely acknowledged as an ideal strain for industrial bio-
oxidations with fantastic yield and productivity. Even 600 g/L xylose can be catalyzed …

Engineering Gluconobacter cerinus CGMCC 1.110 for direct 2-keto-L-gulonic acid production

Z Qin, Y Chen, S Yu, J Chen, J Zhou - Applied Microbiology and …, 2023 - Springer
Gluconobacter is a potential strain for single-step production of 2-keto-L-gulonic acid (2-
KLG), which is the direct precursor of vitamin C. Three dehydrogenases, namely, sorbitol …

Efficient 1,3-dihydroxyacetone biosynthesis in Gluconobacter oxydans using metabolic engineering and a fed-batch strategy

W Zeng, X Shan, L Liu, J Zhou - Bioresources and Bioprocessing, 2022 - Springer
Dihydroxyacetone (DHA) is a commercially important chemical and widely used in
cosmetics, pharmaceuticals, and food industries as it prevents excessive water evaporation …

High Efficiency Rare Earth Element Biomining with Systems Biology Guided Engineering of Gluconobacter oxydans

AM Schmitz, B Pian, S Marecos, M Wu, M Holycross… - bioRxiv, 2023 - biorxiv.org
The global demand for critical rare earth elements (REE) is rising with the increase in
demand for sustainable energy technologies like wind turbines,, electric vehicles,, and high …

[HTML][HTML] Type I CRISPR-Cas-mediated microbial gene editing and regulation

Z Xu, S Chen, W Wu, Y Wen, H Cao - AIMS microbiology, 2023 - ncbi.nlm.nih.gov
There are six major types of CRISPR-Cas systems that provide adaptive immunity in
bacteria and archaea against invasive genetic elements. The discovery of CRISPR-Cas …

Optimizing enzyme properties to enhance dihydroxyacetone production via methylglyoxal biosensor development

K Zhang, M Li, J Wang, G Huang, K Ma, J Peng… - Microbial Cell …, 2024 - Springer
Background Dihydroxyacetone (DHA) stands as a crucial chemical material extensively
utilized in the cosmetics industry. DHA production through the dephosphorylation of …

[HTML][HTML] CRISPR/Cpf1–FOKI-induced gene editing in Gluconobacter oxydans

X Wang, D Li, Z Qin, J Chen, J Zhou - Synthetic and Systems Biotechnology, 2024 - Elsevier
Gluconobacter oxydans is an important Gram-negative industrial microorganism that
produces vitamin C and other products due to its efficient membrane-bound dehydrogenase …

[PDF][PDF] CRISPRe: An innate transcriptional enhancer for endogenous genes in CRISPR-Cas immunity

D Li, Y Chen, F Huang, J Wang, X Li, Y Yang - Iscience, 2023 - cell.com
CRISPR-Cas system has been repurposed to the promising strategy of CRISPR-based
transcriptional interference/activation (CRISPRi/CRISPRa) without eliciting DNA breaks that …

Gluconobacter oxydans Knockout Collection Finds Improved Rare Earth Element Extraction

AM Schmitz, B Pian, S Medin, MC Reid, M Wu, E Gazel… - BioRxiv, 2021 - biorxiv.org
Rare earth elements (REE) are critical components of our technological society and
essential for renewable energy technologies. Traditional thermochemical processes to …