[HTML][HTML] Strategies to overcome the main challenges of the use of CRISPR/Cas9 as a replacement for cancer therapy

MF Rasul, BM Hussen, A Salihi, BS Ismael, PJ Jalal… - Molecular Cancer, 2022 - Springer
CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats-associated protein
9) shows the opportunity to treat a diverse array of untreated various genetic and …

An Overview of Methods and Tools for Transfection of Eukaryotic Cells in vitro

A Fus-Kujawa, P Prus, K Bajdak-Rusinek… - … in bioengineering and …, 2021 - frontiersin.org
Transfection is a powerful analytical tool enabling studies of gene products and functions in
eukaryotic cells. Successful delivery of genetic material into cells depends on DNA quantity …

Viral vectors in gene therapy: Where do we stand in 2023?

K Lundstrom - Viruses, 2023 - mdpi.com
Viral vectors have been used for a broad spectrum of gene therapy for both acute and
chronic diseases. In the context of cancer gene therapy, viral vectors expressing anti-tumor …

Adenoviral vector-based vaccine platform for COVID-19: Current status

VP Chavda, R Bezbaruah, D Valu, B Patel, A Kumar… - Vaccines, 2023 - mdpi.com
The coronavirus disease (COVID-19) breakout had an unimaginable worldwide effect in the
21st century, claiming millions of lives and putting a huge burden on the global economy …

[HTML][HTML] Novel vectors and approaches for gene therapy in liver diseases

S Maestro, ND Weber, N Zabaleta, R Aldabe… - JHEP Reports, 2021 - Elsevier
Gene therapy is becoming an increasingly valuable tool to treat many genetic diseases with
no or limited treatment options. This is the case for hundreds of monogenic metabolic …

Viral Vectors for the in Vivo Delivery of CRISPR Components: Advances and Challenges

M Asmamaw Mengstie - Frontiers in Bioengineering and …, 2022 - frontiersin.org
The Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR) and its
accompanying protein (Cas9) are now the most effective, efficient, and precise genome …

Appraisal for the potential of viral and nonviral vectors in gene therapy: A review

MH Butt, M Zaman, A Ahmad, R Khan, TH Mallhi… - Genes, 2022 - mdpi.com
Over the past few decades, gene therapy has gained immense importance in medical
research as a promising treatment strategy for diseases such as cancer, AIDS, Alzheimer's …

Targeting miRNA by CRISPR/Cas in cancer: advantages and challenges

BM Hussen, MF Rasul, SR Abdullah, HJ Hidayat… - Military Medical …, 2023 - Springer
Clustered regulatory interspaced short palindromic repeats (CRISPR) has changed
biomedical research and provided entirely new models to analyze every aspect of …

Significance of preexisting vector immunity and activation of innate responses for adenoviral vector-based therapy

WC Wang, EE Sayedahmed, SK Mittal - Viruses, 2022 - mdpi.com
An adenoviral (AdV)-based vector system is a promising platform for vaccine development
and gene therapy applications. Administration of an AdV vector elicits robust innate …

Oncolytic virotherapy in glioma tumors

S Rius-Rocabert, N García-Romero, A García… - International Journal of …, 2020 - mdpi.com
Glioma tumors are one of the most devastating cancer types. Glioblastoma is the most
advanced stage with the worst prognosis. Current therapies are still unable to provide an …