Inteins catalyze a post-translational modification known as protein splicing, where the intein
removes itself from a precursor protein and concomitantly ligates the flanking protein …

Intein-mediated protein splicing has become an essential tool in modern biotechnology.
Fundamental progress in the structure and catalytic strategies of cis-and trans-splicing …

Individual proteins can now often be modified with atomic precision, but there are still major
obstacles to connecting proteins into larger assemblies. To direct protein assembly, ideally …

Chemically modified proteins are invaluable tools for studying the molecular details of
biological processes, and they also hold great potential as new therapeutic agents. Several …

Split inteins are a class of naturally occurring proteins that carry out protein splicing in trans.
The chemical mechanism of protein trans-splicing is well-understood and has been …

Split inteins play an important role in modern protein semisynthesis techniques. These
naturally occurring protein splicing domains can be used for in vitro and in vivo protein …

The rational design of supramolecular assemblies aims to generate complex systems based
on the simple information encoded in the chemical structure. Programmable molecules such …

This timely, one-stop reference is the first on an emerging and interdisciplinary topic.
Covering both established and recently developed ligation chemistries, the book is divided …

Ubiquitylation of histone H2B at lysine 120 (H2B-Ub) plays a critical role in transcriptional
elongation, chromatin conformation, as well as the regulation of specific histone H3 …

The Trojan exon method, which makes use of intronically inserted T2A-Gal4 cassettes, has
been widely used in Drosophila to create thousands of gene-specific Gal4 driver lines …