In vivo the poly (A) signal not only directs 3′-end processing but also controls the rate and
extent of transcription. Thus, upon crossing the poly (A) signal RNA polymerase II first …

Eukaryotic cells have a surveillance mechanism that identifies aberrantly processed pre-
mRNAs and prevents their flow to the cytoplasm by tethering them near the site of …

Splicing can occur co-transcriptionally. What happens when the splicing reaction lags after
the completed transcriptional process? We found that elongation rates are independent of …

The Cleavage stimulation Factor (CstF) complex is composed of three subunits and is
essential for pre-mRNA 3′-end processing. CstF recognizes U and G/U-rich cis-acting RNA …

BRCA1 associated ring domain protein 1 (BARD1) is a tumor suppressor protein having a
wide role in cellular processes like cell-cycle checkpoint, DNA damage repair and …

Human immunodeficiency virus type 1 (HIV-1) transcription is regulated by the viral Tat
protein, which relieves a block to elongation by recruiting an elongation factor, P-TEFb, to …

Transcription termination is mechanistically coupled to pre-mRNA 3′ end formation to
prevent transcription much beyond the gene 3′ end. C. elegans, however, engages in …

A useful method for studying the function of the mammalian RNA polymerase II takes
advantage of the extreme sensitivity of its largest subunit, Rpb1, to α-amanitin. Mutations of …

RNA polymerases (RNAPs) are among the most important cellular enzymes. They are
present in all living organisms from Bacteria and Archaea to Eukarya and are responsible for …

Polyadenylation is a key step in mRNA maturation and leads to the sequential addition of
adenosines forming a poly (A) tail, which is important in mRNA stability, nuclear export …