Genome editing with CRISPR–Cas nucleases, base editors, transposases and prime editors
The development of new CRISPR–Cas genome editing tools continues to drive major
advances in the life sciences. Four classes of CRISPR–Cas-derived genome editing agents …
advances in the life sciences. Four classes of CRISPR–Cas-derived genome editing agents …
Base editing: advances and therapeutic opportunities
EM Porto, AC Komor, IM Slaymaker… - Nature Reviews Drug …, 2020 - nature.com
Base editing—the introduction of single-nucleotide variants (SNVs) into DNA or RNA in
living cells—is one of the most recent advances in the field of genome editing. As around …
living cells—is one of the most recent advances in the field of genome editing. As around …
Base editing: precision chemistry on the genome and transcriptome of living cells
RNA-guided programmable nucleases from CRISPR systems generate precise breaks in
DNA or RNA at specified positions. In cells, this activity can lead to changes in DNA …
DNA or RNA at specified positions. In cells, this activity can lead to changes in DNA …
CRISPR-Cas guides the future of genetic engineering
The diversity, modularity, and efficacy of CRISPR-Cas systems are driving a
biotechnological revolution. RNA-guided Cas enzymes have been adopted as tools to …
biotechnological revolution. RNA-guided Cas enzymes have been adopted as tools to …
CRISPR/Cas genome editing and precision plant breeding in agriculture
K Chen, Y Wang, R Zhang, H Zhang… - Annual review of plant …, 2019 - annualreviews.org
Enhanced agricultural production through innovative breeding technology is urgently
needed to increase access to nutritious foods worldwide. Recent advances in CRISPR/Cas …
needed to increase access to nutritious foods worldwide. Recent advances in CRISPR/Cas …
[HTML][HTML] CRISPR/Cas system: recent advances and future prospects for genome editing
H Manghwar, K Lindsey, X Zhang, S Jin - Trends in plant science, 2019 - cell.com
Genome editing (GE) has revolutionized biological research through the new ability to
precisely edit the genomes of living organisms. In recent years, various GE tools have been …
precisely edit the genomes of living organisms. In recent years, various GE tools have been …
Improving cytidine and adenine base editors by expression optimization and ancestral reconstruction
Base editors enable targeted single-nucleotide conversions in genomic DNA. Here we show
that expression levels are a bottleneck in base-editing efficiency. We optimize cytidine (BE4) …
that expression levels are a bottleneck in base-editing efficiency. We optimize cytidine (BE4) …
[HTML][HTML] Expanded base editing in rice and wheat using a Cas9-adenosine deaminase fusion
Nucleotide base editors in plants have been limited to conversion of cytosine to thymine.
Here, we describe a new plant adenine base editor based on an evolved tRNA adenosine …
Here, we describe a new plant adenine base editor based on an evolved tRNA adenosine …
[HTML][HTML] Applications and potential of genome editing in crop improvement
Genome-editing tools provide advanced biotechnological techniques that enable the
precise and efficient targeted modification of an organism's genome. Genome-editing …
precise and efficient targeted modification of an organism's genome. Genome-editing …
Efficient C-to-T base editing in plants using a fusion of nCas9 and human APOBEC3A
Base editors (BEs) have been used to create C-to-T substitutions in various organisms.
However, editing with rat APOBEC1-based BE3 is limited to a 5-nt sequence editing window …
However, editing with rat APOBEC1-based BE3 is limited to a 5-nt sequence editing window …