[HTML][HTML] The CRISPR-Cas toolbox and gene editing technologies
G Liu, Q Lin, S Jin, C Gao - Molecular cell, 2022 - cell.com
The emergence of CRISPR-Cas systems has accelerated the development of gene editing
technologies, which are widely used in the life sciences. To improve the performance of …
technologies, which are widely used in the life sciences. To improve the performance of …
Adeno-associated virus toolkit to target diverse brain cells
RC Challis, S Ravindra Kumar, X Chen… - Annual review of …, 2022 - annualreviews.org
Recombinant adeno-associated viruses (AAVs) are commonly used gene delivery vehicles
for neuroscience research. They have two engineerable features: the capsid (outer protein …
for neuroscience research. They have two engineerable features: the capsid (outer protein …
Fanzor is a eukaryotic programmable RNA-guided endonuclease
RNA-guided systems, which use complementarity between a guide RNA and target nucleic
acid sequences for recognition of genetic elements, have a central role in biological …
acid sequences for recognition of genetic elements, have a central role in biological …
An engineered hypercompact CRISPR-Cas12f system with boosted gene-editing activity
Compact CRISPR-Cas systems offer versatile treatment options for genetic disorders, but
their application is often limited by modest gene-editing activity. Here we present …
their application is often limited by modest gene-editing activity. Here we present …
Comprehensive assessment of miniature CRISPR-Cas12f nucleases for gene disruption
Because of their small size, the recently developed CRISPR-Cas12f nucleases can be
effectively packaged into adeno-associated viruses for gene therapy. However, a systematic …
effectively packaged into adeno-associated viruses for gene therapy. However, a systematic …
Evolutionary mining and functional characterization of TnpB nucleases identify efficient miniature genome editors
G Xiang, Y Li, J Sun, Y Huo, S Cao, Y Cao, Y Guo… - Nature …, 2023 - nature.com
As the evolutionary ancestor of Cas12 nuclease, the transposon (IS200/IS605)-encoded
TnpB proteins act as compact RNA-guided DNA endonucleases. To explore their …
TnpB proteins act as compact RNA-guided DNA endonucleases. To explore their …
A highly specific CRISPR-Cas12j nuclease enables allele-specific genome editing
Y Wang, T Qi, J Liu, Y Yang, Z Wang, Y Wang… - Science …, 2023 - science.org
The CRISPR-Cas system can treat autosomal dominant diseases by nonhomologous end
joining (NHEJ) gene disruption of mutant alleles. However, many single-nucleotide …
joining (NHEJ) gene disruption of mutant alleles. However, many single-nucleotide …
Cas12n nucleases, early evolutionary intermediates of type V CRISPR, comprise a distinct family of miniature genome editors
W Chen, J Ma, Z Wu, Z Wang, H Zhang, W Fu, D Pan… - Molecular cell, 2023 - cell.com
Type V CRISPR-associated systems (Cas) 12 family nucleases are considered to have
evolved from transposon-associated TnpB, and several of these nucleases have been …
evolved from transposon-associated TnpB, and several of these nucleases have been …
A critical review: recent advancements in the use of CRISPR/Cas9 technology to enhance crops and alleviate global food crises
Genome editing (GE) has revolutionized the biological sciences by creating a novel
approach for manipulating the genomes of living organisms. Many tools have been …
approach for manipulating the genomes of living organisms. Many tools have been …
[HTML][HTML] An AsCas12f-based compact genome-editing tool derived by deep mutational scanning and structural analysis
T Hino, SN Omura, R Nakagawa, T Togashi… - Cell, 2023 - cell.com
SpCas9 and AsCas12a are widely utilized as genome-editing tools in human cells.
However, their relatively large size poses a limitation for delivery by cargo-size-limited …
However, their relatively large size poses a limitation for delivery by cargo-size-limited …