TET-mediated active DNA demethylation: mechanism, function and beyond
In mammals, DNA methylation in the form of 5-methylcytosine (5mC) can be actively
reversed to unmodified cytosine (C) through TET dioxygenase-mediated oxidation of 5mC to …
reversed to unmodified cytosine (C) through TET dioxygenase-mediated oxidation of 5mC to …
Mapping the epigenetic modifications of DNA and RNA
Over 17 and 160 types of chemical modifications have been identified in DNA and RNA,
respectively. The interest in understanding the various biological functions of DNA and RNA …
respectively. The interest in understanding the various biological functions of DNA and RNA …
Genome-wide detection of cytosine methylation by single molecule real-time sequencing
5-Methylcytosine (5mC) is an important type of epigenetic modification. Bisulfite sequencing
(BS-seq) has limitations, such as severe DNA degradation. Using single molecule real-time …
(BS-seq) has limitations, such as severe DNA degradation. Using single molecule real-time …
[HTML][HTML] Nucleic acid modifications in regulation of gene expression
Nucleic acids carry a wide range of different chemical modifications. In contrast to previous
views that these modifications are static and only play fine-tuning functions, recent research …
views that these modifications are static and only play fine-tuning functions, recent research …
5-Formylcytosine can be a stable DNA modification in mammals
M Bachman, S Uribe-Lewis, X Yang… - Nature chemical …, 2015 - nature.com
Formylcytosine (5fC) is a rare base found in mammalian DNA and thought to be involved in
active DNA demethylation. Here, we show that developmental dynamics of 5fC levels in …
active DNA demethylation. Here, we show that developmental dynamics of 5fC levels in …
[HTML][HTML] TET family dioxygenases and DNA demethylation in stem cells and cancers
The methylation of cytosine and subsequent oxidation constitutes a fundamental epigenetic
modification in mammalian genomes, and its abnormalities are intimately coupled to various …
modification in mammalian genomes, and its abnormalities are intimately coupled to various …
[HTML][HTML] Effects of cytosine modifications on DNA flexibility and nucleosome mechanical stability
Cytosine can undergo modifications, forming 5-methylcytosine (5-mC) and its oxidized
products 5-hydroxymethylcytosine (5-hmC), 5-formylcytosine (5-fC) and 5-carboxylcytosine …
products 5-hydroxymethylcytosine (5-hmC), 5-formylcytosine (5-fC) and 5-carboxylcytosine …
[HTML][HTML] Single-cell 5-formylcytosine landscapes of mammalian early embryos and ESCs at single-base resolution
Active DNA demethylation in mammals involves ten-eleven translocation (TET) family
protein-mediated oxidation of 5-methylcytosine (5mC). However, base-resolution …
protein-mediated oxidation of 5-methylcytosine (5mC). However, base-resolution …
[HTML][HTML] A highly sensitive and robust method for genome-wide 5hmC profiling of rare cell populations
We present a highly sensitive and selective chemical labeling and capture approach for
genome-wide profiling of 5-hydroxylmethylcytosine (5hmC) using DNA isolated from∼ …
genome-wide profiling of 5-hydroxylmethylcytosine (5hmC) using DNA isolated from∼ …
[HTML][HTML] Subtraction-free and bisulfite-free specific sequencing of 5-methylcytosine and its oxidized derivatives at base resolution
Although various methods have been developed for sequencing cytosine modifications, it is
still challenging for specific and quantitative sequencing of individual modification at base …
still challenging for specific and quantitative sequencing of individual modification at base …