Low-cost biosensor technologies for rapid detection of COVID-19 and future pandemics

WR de Araujo, H Lukas, MDT Torres, W Gao… - ACS …, 2024 - ACS Publications
Many systems have been designed for the detection of SARS-CoV-2, which is the virus that
causes COVID-19. SARS-CoV-2 is readily transmitted, resulting in the rapid spread of …

COVID-19 diagnosis: a comprehensive review of the RT-qPCR method for detection of SARS-CoV-2

D Dutta, S Naiyer, S Mansuri, N Soni, V Singh, KH Bhat… - Diagnostics, 2022 - mdpi.com
The world is grappling with the coronavirus disease 2019 (COVID-19) pandemic, the
causative agent of which is severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) …

The future of point-of-care nucleic acid amplification diagnostics after COVID-19: time to walk the walk

J García-Bernalt Diego, P Fernández-Soto… - International Journal of …, 2022 - mdpi.com
Since the onset of the COVID-19 pandemic, over 610 million cases have been diagnosed
and it has caused over 6.5 million deaths worldwide. The crisis has forced the scientific …

Low-temperature loop-mediated isothermal amplification operating at physiological temperature

D Nam, S Kim, JH Kim, S Lee, D Kim, J Son, D Kim… - Biosensors, 2023 - mdpi.com
Loop-mediated isothermal amplification (LAMP) is one of the most widely used isothermal
amplification technologies in molecular diagnostics. However, LAMP operates at a high …

Rapid and sensitive on-site detection of SARS-CoV-2 RNA from environmental surfaces using portable laboratory devices

K Kitamura, MK Ueno, H Yoshida - Microbiology Spectrum, 2023 - Am Soc Microbiol
Monitoring severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA on
inanimate surfaces plays a crucial role in environmental surveillance, enabling the indirect …

A Molecular Lateral Flow Assay for SARS-CoV-2 Quantitative Detection

P Maglaras, I Lilis, F Paliogianni, V Bravou… - Biosensors, 2022 - mdpi.com
Since the onset of the SARS-CoV-2 pandemic, several COVID-19 detection methods, both
commercially available and in the lab, have been developed using different biomolecules as …

The potential of tailed amplicons for SARS-CoV-2 detection in Nucleic Acid Lateral Flow Assays

JM Vindeirinho, R Oliveira, E Pinho, R Guiomar… - Plos one, 2024 - journals.plos.org
Nucleic Acid Lateral Flow Assays (NALFAs) are a promising solution for the point-of-care
detection of viruses like SARS-CoV-2. However, they show some drawbacks, such as the …

Detection of specific RNA targets by multimerization

AR Sakhabutdinova, AV Chemeris… - Biochemistry (Moscow …, 2023 - Springer
Detection of specific RNA targets via amplification-mediated techniques is widely used in
fundamental studies and medicine due to essential role of RNA in transfer of genetic …

Reverse transcriptase-free detection of viral RNA using Hemo KlenTaq DNA polymerase

AR Sakhabutdinova, RR Gazizov, AV Chemeris… - Analytical …, 2022 - Elsevier
COVID-19 pandemic highlighted the demand for the fast and reliable detection of viral RNA.
Although various methods for RNA amplification and detection have been proposed, some …

Flexible upscaling of laboratory PCR testing capacity at the Robert Koch Institute during the SARS-CoV-2 pandemic

E Krause, J Michel, A Puyskens, N Hofmann, T Rinner… - Virology Journal, 2023 - Springer
Background Over the course of the COVID-19 pandemic, laboratories worldwide have been
facing an unprecedented increase in demand for PCR testing because of the high …