Comparison of TaqMan and SYBR Green qPCR methods for quantitative gene expression in tung tree tissues

H Cao, JM Shockey - Journal of agricultural and food chemistry, 2012 - ACS Publications
Quantitative real-time-PCR (qPCR) is widely used for gene expression analysis due to its
large dynamic range, tremendous sensitivity, high sequence specificity, little to no …

Selection of Reference Genes for Quantitative Real-Time PCR in Bamboo (Phyllostachys edulis)

C Fan, J Ma, Q Guo, X Li, H Wang, M Lu - PloS one, 2013 - journals.plos.org
Background The Moso bamboo (Phyllostachys edulis) is one of the most important forestry
resources and plays essential ecological roles in southern China. A draft nuclear genome …

Identification and Validation of Reference Genes for Gene Expression Analysis in Schima superba

Z Yang, R Zhang, Z Zhou - Genes, 2021 - mdpi.com
Real-time quantitative PCR (RT-qPCR) is a reliable and high-throughput technique for gene
expression studies, but its accuracy depends on the expression stability of reference genes …

Identification of candidate reference genes in perennial ryegrass for quantitative RT-PCR under various abiotic stress conditions

L Huang, H Yan, X Jiang, G Yin, X Zhang, X Qi… - PloS one, 2014 - journals.plos.org
Background Quantitative real-time reverse-transcriptase PCR (qRT-PCR) is an important
technique for analyzing differences in gene expression due to its sensitivity, accuracy and …

Identification and validation of reference genes for qRT-PCR analyses under different experimental conditions in Allium wallichii

Y Lin, G Liu, Y Rao, B Wang, R Tian, Y Tan… - Journal of Plant …, 2023 - Elsevier
Himalayan onion (Allium wallichii) is a perennial bulbous herb with high ornamental value
and has long been used as traditional medicines in Nepal and China because of the anti …

实时荧光定量PCR 分析中毛果杨内参基因的筛选和验证

苏晓娟, 樊保国, 袁丽钗, 崔秀娜, 卢善发 - 植物学报, 2013 - chinbullbotany.com
实时荧光定量PCR (qRT-PCR) 技术具有高灵敏性, 高保真性和高特异性,
被广泛应用于基因表达的分析. 在数据处理过程中, 选用稳定表达的基因作为内参基因对准确 …

Reference gene selection for qRT-PCR analysis of flower development in Lagerstroemia indica and L. speciosa

T Zheng, Z Chen, Y Ju, H Zhang, M Cai, H Pan… - PloS one, 2018 - journals.plos.org
Quantitative real-time polymerase chain reaction (qRT-PCR) is a prevalent method for gene
expression analysis, depending on the stability of the reference genes for data …

Ascorbate glutathione-dependent H2O2 scavenging is an important process in axillary bud outgrowth in rosebush

A Porcher, V Guérin, F Montrichard, A Lebrec… - Annals of …, 2020 - academic.oup.com
Abstract Background and Aims Branching is an important mechanism of plant shape
establishment and the direct consequence of axillary bud outgrowth. Recently, hydrogen …

DNA profiling, telomere analysis and antioxidant properties as tools for monitoring ex situ seed longevity

M Donà, A Balestrazzi, A Mondoni, G Rossi… - Annals of …, 2013 - academic.oup.com
Abstract Background and Aims The germination test currently represents the most used
method to assess seed viability in germplasm banks, despite the difficulties caused by the …

Identification and Expression Analysis of Glucosinolate Biosynthetic Genes and Estimation of Glucosinolate Contents in Edible Organs of Brassica oleracea …

GE Yi, AHK Robin, K Yang, JI Park, JG Kang, TJ Yang… - Molecules, 2015 - mdpi.com
Glucosinolates are anti-carcinogenic, anti-oxidative biochemical compounds that defend
plants from insect and microbial attack. Glucosinolates are abundant in all cruciferous crops …