Existing at the interface of biology and electronics, living cells have been in use as
biorecognition elements (bioreceptors) in biosensors since the early 1970s. They are an …

Transcription-factor-based biosensors (TFBs) are often used for metabolite detection,
adaptive evolution, and metabolic flux control. However, designing TFBs with superior …

Synthetic biology is the discipline of engineering application-driven biological functionalities
that were not evolved by nature. Early breakthroughs of cell engineering, which were based …

In the first wave of synthetic biology, genetic elements, combined into simple circuits, are
used to control individual cellular functions. In the second wave of synthetic biology, the …

Methods to regulate gene expression programs in bacterial cells are limited by the absence
of effective gene activators. To address this challenge, we have developed synthetic …

Proteins found in nature have traditionally been the most frequently used biocatalysts to
produce numerous natural products ranging from commodity chemicals to pharmaceuticals …

Low yield and low titer of natural products are common issues in natural product
biosynthesis through microbial cell factories. One effective way to resolve such bottlenecks …

Metabolic engineering seeks to rewire the metabolic network of cells for the efficient
production of value-added compounds from renewable substrates. However, it remains …

Resveratrol is a plant secondary metabolite with multiple health-beneficial properties.
Microbial production of resveratrol in model microorganisms requires extensive engineering …

Adaptive laboratory evolution (ALE) has served as a historic microbial engineering method
that mimics natural selection to obtain desired microbes. The past decade has witnessed …