Highlights•CRISPR–Cas systems promoted the engineering of non-model bacteria.•Tools
include genome modifications and finely-tuned transcriptional regulation.•CRISPR–Cas …

Microbial biomanufacturing, powered by the advances of synthetic biology, has attracted
growing interest for the production of diverse products. In contrast to conventional microbes …

CRISPR/Cas technologies constitute a powerful tool for genome engineering, yet their use
in non-traditional bacteria depends on host factors or exogenous recombinases, which limits …

The study of growth (of cells, organ-isms or populations) is inherent to all biological
disciplines. The fundamental properties of a biological system or the physiological effects of …

Automation is playing an increasingly significant role in synthetic biology. Groundbreaking
technologies, developed over the past 20 years, have enormously accelerated the …

Many efforts have been put into engineering plants to improve crop yields and stress
tolerance and boost the bioproduction of valuable molecules. Yet, our capabilities are still …

Synthetic biology aims to design or assemble existing bioparts or bio-components for useful
bioproperties. During the past decades, progresses have been made to build delicate …

Pseudomonas chlororaphis is a non‐pathogenic, plant growth‐promoting rhizobacterium
that secretes phenazine compounds with broad‐spectrum antibiotic activity. Currently …

A synthetic biology toolkit, exploiting clustered regularly interspaced short palindromic
repeats (CRISPR) and modified CRISPR-associated protein (Cas) base-editors, was …

Highlights•Combining enzyme engineering and pathway design allows for many methanol
assimilation routes.•Several, reduced products can be synthesized from a mixture of sugar …