微生物合成生物学是一门新兴的交叉学科, 其主要目的是通过改造或创制微生物细胞,
使微生物具有特定的生理功能或生产目标产物, 因此需要高效, 快速, 精准的基因操作工具 …

Shiraia bambusicola can produce a type of hypocrellin, which is applied in antibacterial,
antitumoral, and antiviral areas. Studies on the hypocrellin pathway have not been …

Genome-editing CRISPR-Cas systems, using Cas9 and Cas12a endonucleases, have
improved our ability to precisely edit genomes and control gene expression. We summarize …

Targeted genome editing technology is becoming one of the most important genetic tools
and widely employed in the plant pathology community. In recent years, CRISPR (Clustered …

Genome engineering is a biotechnological approach to precisely modify the genetic code of
a given organism in order to change the context of an existing sequence or to create new …

Background U6 promoters have been used for single guide RNA (sgRNA) transcription in
the clustered regularly interspaced short palindromic repeats/CRISPR-associated protein …

Colletotrichum graminicola causes anthracnose on maize, an economically significant
disease worldwide. To decipher how the pathogen controls its virulence/pathogenicity on …

Although sexual reproduction is widespread in eukaryotes, some fungal species can only
reproduce asexually. In the rice blast fungus Pyricularia (Magnaporthe) oryzae, several …

Research on fungal pathogens with the aim to identify virulence determinants strictly relies
on the generation of defined, recombinant strains, a task that is executed by means of a …

Genetic variation is the stuff of evolution: if there is no variation, there can be no evolution.
This review of fungal genetic variation begins with a survey of its sources and then …