DNA repair pathway choices in CRISPR-Cas9-mediated genome editing
Many clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-
associated protein 9 (Cas9)-based genome editing technologies take advantage of Cas …
associated protein 9 (Cas9)-based genome editing technologies take advantage of Cas …
[HTML][HTML] CRISPR-based genome editing through the lens of DNA repair
Genome editing technologies operate by inducing site-specific DNA perturbations that are
resolved by cellular DNA repair pathways. Products of genome editors include DNA breaks …
resolved by cellular DNA repair pathways. Products of genome editors include DNA breaks …
Advances in genome editing through control of DNA repair pathways
Eukaryotic cells deploy overlapping repair pathways to resolve DNA damage.
Advancements in genome editing take advantage of these pathways to produce permanent …
Advancements in genome editing take advantage of these pathways to produce permanent …
The emerging and uncultivated potential of CRISPR technology in plant science
The application of clustered regularly interspaced short palindromic repeats (CRISPR) for
genetic manipulation has revolutionized life science over the past few years. CRISPR was …
genetic manipulation has revolutionized life science over the past few years. CRISPR was …
[HTML][HTML] Methods favoring homology-directed repair choice in response to CRISPR/Cas9 induced-double strand breaks
H Yang, S Ren, S Yu, H Pan, T Li, S Ge… - International journal of …, 2020 - mdpi.com
Precise gene editing is—or will soon be—in clinical use for several diseases, and more
applications are under development. The programmable nuclease Cas9, directed by a …
applications are under development. The programmable nuclease Cas9, directed by a …
[HTML][HTML] CRISPR-Cas9 genome editing induces megabase-scale chromosomal truncations
G Cullot, J Boutin, J Toutain, F Prat… - Nature …, 2019 - nature.com
CRISPR-Cas9 is a promising technology for genome editing. Here we use Cas9 nuclease-
induced double-strand break DNA (DSB) at the UROS locus to model and correct congenital …
induced double-strand break DNA (DSB) at the UROS locus to model and correct congenital …
Fast and efficient generation of knock-in human organoids using homology-independent CRISPR–Cas9 precision genome editing
CRISPR–Cas9 technology has revolutionized genome editing and is applicable to the
organoid field. However, precise integration of exogenous DNA sequences into human …
organoid field. However, precise integration of exogenous DNA sequences into human …
[HTML][HTML] Self-inactivating, all-in-one AAV vectors for precision Cas9 genome editing via homology-directed repair in vivo
Adeno-associated virus (AAV) vectors are important delivery platforms for therapeutic
genome editing but are severely constrained by cargo limits. Simultaneous delivery of …
genome editing but are severely constrained by cargo limits. Simultaneous delivery of …
Dynamics and competition of CRISPR–Cas9 ribonucleoproteins and AAV donor-mediated NHEJ, MMEJ and HDR editing
YW Fu, XY Dai, WT Wang, ZX Yang… - Nucleic acids …, 2021 - academic.oup.com
Investigations of CRISPR gene knockout editing profiles have contributed to enhanced
precision of editing outcomes. However, for homology-directed repair (HDR) in particular …
precision of editing outcomes. However, for homology-directed repair (HDR) in particular …
RNA–protein interaction detection in living cells
RNA–protein interactions play numerous roles in cellular function and disease. Here we
describe RNA–protein interaction detection (RaPID), which uses proximity-dependent …
describe RNA–protein interaction detection (RaPID), which uses proximity-dependent …