Cell-free biology is the activation of biological processes without the use of intact living cells.
It has been used for more than 50 years across the life sciences as a foundational research …

Cell-free gene expression (CFE) emerged as an alternative approach to living cells for
specific applications in protein synthesis and labelling for structural biology and proteomics …

Cell-free protein synthesis (CFPS) is a platform technology that provides new opportunities
for protein expression, metabolic engineering, therapeutic development, education, and …

In recent years, bacteriophage research has been boosted by a rising interest in using
phage therapy to treat antibiotic-resistant bacterial infections. In addition, there is a desire to …

Owing to rising levels of greenhouse gases in our atmosphere and oceans, climate change
poses significant environmental, economic, and social challenges globally. Technologies …

Cell-free protein synthesis (CFPS) system is a simple, rapid, and sensitive tool that is devoid
of membrane-bound barriers, yet contains all the mandatory substrates, biomolecules, and …

Recent advances in cell-free gene expression (CFE) systems have enabled their use for a
host of synthetic biology applications, particularly for rapid prototyping of genetic circuits and …

Proteins are the main source of drug targets and some of them possess therapeutic potential
themselves. Among them, membrane proteins constitute approximately 50% of the major …

The marine bacterium Vibrio natriegens is the fastest‐growing non‐pathogenic bacterium
known to date and is gaining more and more attention as an alternative chassis organism to …

Highlights•Cell-free systems enable total biosynthesis of complex natural products.•Cell-free
systems are robust to express long biosynthetic gene clusters (> 10 kb).•Cell-free reactions …