Luminescence imaging in biomedical research has allowed new insights into the
architecture of pathological tissues, signalling networks and cell interactions, and is now …

Optical imaging is an indispensable tool in clinical diagnostics and fundamental biomedical
research. Autofluorescence-free optical imaging, which eliminates real-time optical …

Significance: Fluorescence lifetime imaging microscopy (FLIM) is a powerful technique to
distinguish the unique molecular environment of fluorophores. FLIM measures the time a …

Biomedical fluorescence imaging in the second near-infrared (NIR-II, 100–1700 nm) window
provides great potential for visualizing physiological and pathological processes, owing to …

Photoluminescence lifetime imaging of upconverting nanoparticles is increasingly featured
in recent progress in optical thermometry. Despite remarkable advances in …

In the year of 2019, we celebrate the centenary of the publication of the remarkable paper by
Otto Stern and Max Volmer concerning the kinetics analysis of fluorescence quenching …

Single-photon avalanche diode (SPAD) arrays are solid-state detectors that offer imaging
capabilities at the level of individual photons, with unparalleled photon counting and time …

In this Review article, we systematically summarize the design and applications of various
kinds of long-lived emissive probes for bioimaging and biosensing via time-resolved …

Förster or fluorescence resonance energy transfer (FRET) technology and genetically
encoded FRET biosensors provide a powerful tool for visualizing signaling molecules in live …

Stimulated emission depletion (STED) nanoscopy is a promising fluorescence microscopy to
detect unresolvable structures at the nanoscale level and then achieve a superior imaging …