Bacterial inclusion bodies: a treasure trove of bioactive proteins
Recombinant proteins expressed as bacterial inclusion bodies (IBs) are now receiving
tremendous attention for many diverse applications in the areas of industrial and medical …
tremendous attention for many diverse applications in the areas of industrial and medical …
[HTML][HTML] Refolding in the modern biopharmaceutical industry
L Buscajoni, MC Martinetz, M Berkemeyer… - Biotechnology …, 2022 - Elsevier
Inclusion bodies (IBs) often emerge upon overexpression of recombinant proteins in E. coli.
From IBs, refolding is necessary to generate the native protein that can be further purified to …
From IBs, refolding is necessary to generate the native protein that can be further purified to …
[HTML][HTML] Quality comparison of recombinant soluble proteins and proteins solubilized from bacterial inclusion bodies
A López-Cano, P Sicilia, C Gaja, A Arís… - New …, 2022 - Elsevier
Recombinant protein production in bacteria is often accompanied by the formation of
aggregates, known as inclusion bodies (IBs). Although several strategies have been …
aggregates, known as inclusion bodies (IBs). Although several strategies have been …
Solubilization and refolding of inclusion body proteins
Expression of heterologous proteins in E. coli often leads to the formation of protein
aggregates known as inclusion bodies (IBs). Inclusion body aggregates pose a major hurdle …
aggregates known as inclusion bodies (IBs). Inclusion body aggregates pose a major hurdle …
Bioprocessing of recombinant proteins from Escherichia coli inclusion bodies: insights from structure-function relationship for novel applications
The formation of inclusion bodies (IBs) during expression of recombinant therapeutic
proteins using E. coli is a significant hurdle in producing high-quality, safe, and efficacious …
proteins using E. coli is a significant hurdle in producing high-quality, safe, and efficacious …
[HTML][HTML] Molecular attributes associated with refolding of inclusion body proteins using the freeze–thaw method
P Singhvi, J Verma, N Panwar, TQ Wani… - Frontiers in …, 2021 - frontiersin.org
Understanding the structure–function of inclusion bodies (IBs) in the last two decades has
led to the development of several mild solubilization buffers for the improved recovery of …
led to the development of several mild solubilization buffers for the improved recovery of …
State-of-the-art and novel approaches to mild solubilization of inclusion bodies
R Klausser, J Kopp, E Prada Brichtova… - … in Bioengineering and …, 2023 - frontiersin.org
Throughout the twenty-first century, the view on inclusion bodies (IBs) has shifted from
undesired by-products towards a targeted production strategy for recombinant proteins …
undesired by-products towards a targeted production strategy for recombinant proteins …
The establishment and application of indirect 3AB-ELISA for the detection of antibodies against senecavirus A
J Yan, Y Gao, J Li, M Li, C Guo, J Bai, P Jiang - Viruses, 2023 - mdpi.com
Senecavirus A (SVA) is an emerging pathogen that negatively affects the pig industry in
China. Affected animals present vesicular lesions which are indistinguishable from other …
China. Affected animals present vesicular lesions which are indistinguishable from other …
Selecting subpopulations of high-quality protein conformers among conformational mixtures of recombinant bovine mmp-9 solubilized from inclusion bodies
A detailed workflow to analyze the physicochemical characteristics of mammalian matrix
metalloproteinase (MMP-9) protein species obtained from protein aggregates (inclusion …
metalloproteinase (MMP-9) protein species obtained from protein aggregates (inclusion …
Latcripin-7A, derivative of Lentinula edodes C91–3, reduces migration and induces apoptosis, autophagy, and cell cycle arrest at G1 phase in breast cancer cells
Due to the high mortality rate and an increase in breast cancer incidence, it has been
challenging for researchers to come across an effective chemotherapeutic strategy with …
challenging for researchers to come across an effective chemotherapeutic strategy with …