Bacterial inclusion bodies: a treasure trove of bioactive proteins

P Singhvi, A Saneja, S Srichandan, AK Panda - Trends in Biotechnology, 2020 - cell.com
Recombinant proteins expressed as bacterial inclusion bodies (IBs) are now receiving
tremendous attention for many diverse applications in the areas of industrial and medical …

[HTML][HTML] Refolding in the modern biopharmaceutical industry

L Buscajoni, MC Martinetz, M Berkemeyer… - Biotechnology …, 2022 - Elsevier
Inclusion bodies (IBs) often emerge upon overexpression of recombinant proteins in E. coli.
From IBs, refolding is necessary to generate the native protein that can be further purified to …

[HTML][HTML] Quality comparison of recombinant soluble proteins and proteins solubilized from bacterial inclusion bodies

A López-Cano, P Sicilia, C Gaja, A Arís… - New …, 2022 - Elsevier
Recombinant protein production in bacteria is often accompanied by the formation of
aggregates, known as inclusion bodies (IBs). Although several strategies have been …

Solubilization and refolding of inclusion body proteins

P Singhvi, AK Panda - Insoluble Proteins: Methods and Protocols, 2022 - Springer
Expression of heterologous proteins in E. coli often leads to the formation of protein
aggregates known as inclusion bodies (IBs). Inclusion body aggregates pose a major hurdle …

Bioprocessing of recombinant proteins from Escherichia coli inclusion bodies: insights from structure-function relationship for novel applications

K Kachhawaha, S Singh, K Joshi, P Nain… - Preparative …, 2023 - Taylor & Francis
The formation of inclusion bodies (IBs) during expression of recombinant therapeutic
proteins using E. coli is a significant hurdle in producing high-quality, safe, and efficacious …

[HTML][HTML] Molecular attributes associated with refolding of inclusion body proteins using the freeze–thaw method

P Singhvi, J Verma, N Panwar, TQ Wani… - Frontiers in …, 2021 - frontiersin.org
Understanding the structure–function of inclusion bodies (IBs) in the last two decades has
led to the development of several mild solubilization buffers for the improved recovery of …

State-of-the-art and novel approaches to mild solubilization of inclusion bodies

R Klausser, J Kopp, E Prada Brichtova… - … in Bioengineering and …, 2023 - frontiersin.org
Throughout the twenty-first century, the view on inclusion bodies (IBs) has shifted from
undesired by-products towards a targeted production strategy for recombinant proteins …

The establishment and application of indirect 3AB-ELISA for the detection of antibodies against senecavirus A

J Yan, Y Gao, J Li, M Li, C Guo, J Bai, P Jiang - Viruses, 2023 - mdpi.com
Senecavirus A (SVA) is an emerging pathogen that negatively affects the pig industry in
China. Affected animals present vesicular lesions which are indistinguishable from other …

Selecting subpopulations of high-quality protein conformers among conformational mixtures of recombinant bovine mmp-9 solubilized from inclusion bodies

JV Carratalá, L Gifre-Renom, R Roca-Pinilla… - International journal of …, 2021 - mdpi.com
A detailed workflow to analyze the physicochemical characteristics of mammalian matrix
metalloproteinase (MMP-9) protein species obtained from protein aggregates (inclusion …

Latcripin-7A, derivative of Lentinula edodes C91–3, reduces migration and induces apoptosis, autophagy, and cell cycle arrest at G1 phase in breast cancer cells

SRU Din, M Zhong, MA Nisar, MZ Saleem… - Applied Microbiology …, 2020 - Springer
Due to the high mortality rate and an increase in breast cancer incidence, it has been
challenging for researchers to come across an effective chemotherapeutic strategy with …