Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening
Forward genetic screens are powerful tools for the unbiased discovery and functional
characterization of specific genetic elements associated with a phenotype of interest …
characterization of specific genetic elements associated with a phenotype of interest …
CRISPR–Act3. 0 for highly efficient multiplexed gene activation in plants
RNA-guided CRISPR activation (CRISPRa) systems have been developed in plants.
However, the simultaneous activation of multiple genes remains challenging. Here, we …
However, the simultaneous activation of multiple genes remains challenging. Here, we …
Increasing the specificity of CRISPR systems with engineered RNA secondary structures
CRISPR (clustered regularly interspaced short palindromic repeat) systems have been
broadly adopted for basic science, biotechnology, and gene and cell therapy. In some …
broadly adopted for basic science, biotechnology, and gene and cell therapy. In some …
Compact and highly active next-generation libraries for CRISPR-mediated gene repression and activation
We recently found that nucleosomes directly block access of CRISPR/Cas9 to DNA. Here,
we build on this observation with a comprehensive algorithm that incorporates chromatin …
we build on this observation with a comprehensive algorithm that incorporates chromatin …
CRISPR/Cas9-based genome editing for disease modeling and therapy: challenges and opportunities for nonviral delivery
Genome editing offers promising solutions to genetic disorders by editing DNA sequences
or modulating gene expression. The clustered regularly interspaced short palindromic …
or modulating gene expression. The clustered regularly interspaced short palindromic …
Beyond editing: repurposing CRISPR–Cas9 for precision genome regulation and interrogation
AA Dominguez, WA Lim, LS Qi - Nature reviews Molecular cell biology, 2016 - nature.com
The bacterial CRISPR–Cas9 system has emerged as a multifunctional platform for
sequence-specific regulation of gene expression. This Review describes the development of …
sequence-specific regulation of gene expression. This Review describes the development of …
Repurposing the CRISPR-Cas9 system for targeted DNA methylation
Epigenetic studies relied so far on correlations between epigenetic marks and gene
expression pattern. Technologies developed for epigenome editing now enable direct study …
expression pattern. Technologies developed for epigenome editing now enable direct study …
In vivo target gene activation via CRISPR/Cas9-mediated trans-epigenetic modulation
HK Liao, F Hatanaka, T Araoka, P Reddy, MZ Wu, Y Sui… - Cell, 2017 - cell.com
Current genome-editing systems generally rely on inducing DNA double-strand breaks
(DSBs). This may limit their utility in clinical therapies, as unwanted mutations caused by …
(DSBs). This may limit their utility in clinical therapies, as unwanted mutations caused by …
Highly specific epigenome editing by CRISPR-Cas9 repressors for silencing of distal regulatory elements
PI Thakore, AM D'ippolito, L Song, A Safi… - Nature …, 2015 - nature.com
Epigenome editing with the CRISPR (clustered, regularly interspaced, short palindromic
repeats)-Cas9 platform is a promising technology for modulating gene expression to direct …
repeats)-Cas9 platform is a promising technology for modulating gene expression to direct …
CRISPR/Cas9 in genome editing and beyond
The Cas9 protein (CRISPR-associated protein 9), derived from type II CRISPR (clustered
regularly interspaced short palindromic repeats) bacterial immune systems, is emerging as a …
regularly interspaced short palindromic repeats) bacterial immune systems, is emerging as a …