Methods for profiling genes at the single-cell level have revolutionized our ability to study
several biological processes and systems including development, differentiation, response …

Abstract Machine learning (ML) is being ubiquitously incorporated into everyday products
such as Internet search, email spam filters, product recommendations, image classification …

Oral squamous cell carcinoma (OSCC) is a lethal disease whose incidence is increasing.
Epidemiologic studies demonstrate an association between periodontitis and oral cancer …

With the growing importance of microbiome research, there is increasing evidence that host
variation in microbial communities is associated with overall host health. Advancement in …

Hepatocellular carcinoma (HCC) is among the leading causes of cancer‐related death.
Despite the advances in diagnosis and management of HCC, the biology of this tumor …

Most cancer deaths are caused by haematogenous metastatic spread and subsequent
growth of tumour cells at distant organs. Disseminating tumour cells present in the …

Background Accuracy in quantitative real-time RT-PCR is dependent on high quality RNA,
consistent cDNA synthesis, and validated stable reference genes for data normalization …

Background RT-qPCR is a preferred method for rapid and reliable quantification of gene
expression studies. Appropriate application of RT-qPCR in such studies requires the use of …

Drosophila melanogaster is one of the most important genetic models and techniques such
as reverse transcription quantitative real-time PCR (RT-qPCR) are being employed …

Background Real-time quantitative PCR (qPCR) is a method for rapid and reliable
quantification of mRNA transcription. Internal standards such as reference genes are used to …