Gram‐positive bacterial extracellular membrane vesicles (EVs) have been drawing more
attention in recent years. However, mechanistic insights are still lacking on how EVs are …

The analysis of proteins in biological samples is highly desirable, given their connection to
myriad biological functions and disease states, as well as the growing interest in the …

Cardiovascular disease is the leading cause of death, with atherosclerosis the major
underlying cause. While often asymptomatic for decades, atherosclerotic plaque …

Mitochondria are critical modulators of antiviral tolerance through the release of
mitochondrial RNA and DNA (mtDNA and mtRNA) fragments into the cytoplasm after …

Human plasma is a rich source of biomedical information and biomarkers. However, the
enormous dynamic range of plasma proteins limits its accessibility to mass spectrometric …

Abstract Polycomb repressive complex 2 (PRC2) mediates epigenetic silencing of target
genes in animals and plants. In Arabidopsis, PRC2 is required for the cold-induced …

Performing large-scale plasma proteome profiling is challenging due to limitations imposed
by lengthy preparation and instrument time. We present a fully automated multiplexed …

Trypsin is the protease of choice in bottom-up proteomics. However, its application can be
limited by the amino acid composition of target proteins and the pH of the digestion solution …

Background Microbe-microbe interactions between members of the plant rhizosphere are
important but remain poorly understood. A more comprehensive understanding of the …

Selecting a sample preparation strategy for mass spectrometry-based proteomics is critical
to the success of quantitative workflows. Here we present a universal, solid-phase protein …