Genome editing in bacteria: CRISPR-Cas and beyond
RD Arroyo-Olarte, R Bravo Rodriguez, E Morales-Ríos - Microorganisms, 2021 - mdpi.com
Genome editing in bacteria encompasses a wide array of laborious and multi-step methods
such as suicide plasmids. The discovery and applications of clustered regularly interspaced …
such as suicide plasmids. The discovery and applications of clustered regularly interspaced …
Insertion sequence transposition inactivates CRISPR-Cas immunity
Y Sheng, H Wang, Y Ou, Y Wu, W Ding, M Tao… - Nature …, 2023 - nature.com
CRISPR-Cas immunity systems safeguard prokaryotic genomes by inhibiting the invasion of
mobile genetic elements. Here, we screened prokaryotic genomic sequences and identified …
mobile genetic elements. Here, we screened prokaryotic genomic sequences and identified …
High‐efficiency delivery of CRISPR‐Cas9 by engineered probiotics enables precise microbiome editing
K Neil, N Allard, P Roy, F Grenier… - Molecular systems …, 2021 - embopress.org
Antibiotic resistance threatens our ability to treat infectious diseases, spurring interest in
alternative antimicrobial technologies. The use of bacterial conjugation to deliver CRISPR …
alternative antimicrobial technologies. The use of bacterial conjugation to deliver CRISPR …
Genome-wide CRISPR-dCas9 screens in E. coli identify essential genes and phage host factors
High-throughput genetic screens are powerful methods to identify genes linked to a given
phenotype. The catalytic null mutant of the Cas9 RNA-guided nuclease (dCas9) can be …
phenotype. The catalytic null mutant of the Cas9 RNA-guided nuclease (dCas9) can be …
[HTML][HTML] Phage-delivered CRISPR-Cas9 for strain-specific depletion and genomic deletions in the gut microbiome
KN Lam, P Spanogiannopoulos, P Soto-Perez… - Cell Reports, 2021 - cell.com
Mechanistic insights into the role of the human microbiome in the predisposition to and
treatment of disease are limited by the lack of methods to precisely add or remove microbial …
treatment of disease are limited by the lack of methods to precisely add or remove microbial …
Deaminase-mediated multiplex genome editing in Escherichia coli
S Banno, K Nishida, T Arazoe, H Mitsunobu… - Nature …, 2018 - nature.com
In eukaryotes, the CRISPR–Cas9 system has now been widely used as a revolutionary
genome engineering tool,. However, in prokaryotes, the use of nuclease-mediated genome …
genome engineering tool,. However, in prokaryotes, the use of nuclease-mediated genome …
CRISPR/Cas9-coupled recombineering for metabolic engineering of Corynebacterium glutamicum
Genome engineering of Corynebacterium glutamicum, an important industrial
microorganism for amino acids production, currently relies on random mutagenesis and …
microorganism for amino acids production, currently relies on random mutagenesis and …
CRISPR-Cas-based antimicrobials: design, challenges, and bacterial mechanisms of resistance
A Mayorga-Ramos, J Zúñiga-Miranda… - ACS Infectious …, 2023 - ACS Publications
The emergence of antibiotic-resistant bacterial strains is a source of public health concern
across the globe. As the discovery of new conventional antibiotics has stalled significantly …
across the globe. As the discovery of new conventional antibiotics has stalled significantly …
CRISPR-Cas12a-assisted recombineering in bacteria
MY Yan, HQ Yan, GX Ren, JP Zhao… - Applied and …, 2017 - Am Soc Microbiol
Clustered regularly interspaced short palindromic repeat (CRISPR)-Cas12a (Cpf1) has
emerged as an effective genome editing tool in many organisms. Here, we developed and …
emerged as an effective genome editing tool in many organisms. Here, we developed and …
The mechanism of bacterial resistance and potential bacteriostatic strategies
F Zhang, W Cheng - Antibiotics, 2022 - mdpi.com
Bacterial drug resistance is rapidly developing as one of the greatest threats to human
health. Bacteria will adopt corresponding strategies to crack the inhibitory effect of antibiotics …
health. Bacteria will adopt corresponding strategies to crack the inhibitory effect of antibiotics …