Genome editing in bacteria encompasses a wide array of laborious and multi-step methods
such as suicide plasmids. The discovery and applications of clustered regularly interspaced …

CRISPR-Cas immunity systems safeguard prokaryotic genomes by inhibiting the invasion of
mobile genetic elements. Here, we screened prokaryotic genomic sequences and identified …

Antibiotic resistance threatens our ability to treat infectious diseases, spurring interest in
alternative antimicrobial technologies. The use of bacterial conjugation to deliver CRISPR …

High-throughput genetic screens are powerful methods to identify genes linked to a given
phenotype. The catalytic null mutant of the Cas9 RNA-guided nuclease (dCas9) can be …

Mechanistic insights into the role of the human microbiome in the predisposition to and
treatment of disease are limited by the lack of methods to precisely add or remove microbial …

In eukaryotes, the CRISPR–Cas9 system has now been widely used as a revolutionary
genome engineering tool,. However, in prokaryotes, the use of nuclease-mediated genome …

Genome engineering of Corynebacterium glutamicum, an important industrial
microorganism for amino acids production, currently relies on random mutagenesis and …

The emergence of antibiotic-resistant bacterial strains is a source of public health concern
across the globe. As the discovery of new conventional antibiotics has stalled significantly …

Clustered regularly interspaced short palindromic repeat (CRISPR)-Cas12a (Cpf1) has
emerged as an effective genome editing tool in many organisms. Here, we developed and …

Bacterial drug resistance is rapidly developing as one of the greatest threats to human
health. Bacteria will adopt corresponding strategies to crack the inhibitory effect of antibiotics …