Klebsiella pneumoniae is a promising industrial microorganism as well as a major human
pathogen. The recent emergence of carbapenem-resistant K. pneumoniae has posed a …

CRISPR/Cas9 technology has become the most examined gene editing technology in
recent years due to its simple design, yet low cost, high efficiency, and simple operation …

The Host-microbiome interactions that exist inside the gut microbiota operate in a synergistic
and abnormal manner. Additionally, the normal homeostasis and functioning of gut …

The development of antibiotic resistance in bacteria is a major public health threat. Infection
rates of resistant pathogens continue to rise against nearly all antimicrobials, which has led …

While adoption of single‐stranded DNA recombineering techniques has greatly eased
genetic design of the platform strain Pseudomonas putida KT2440, available methods still …

Background Corynebacterium glutamicum is an important industrial workhorse and
advanced genetic engineering tools are urgently demanded. Recently, the clustered …

Throughout the decades of its history, the advances in bacteria-based bio-industries have
coincided with great leaps in strain engineering technologies. Recently unveiled clustered …

In metabolic engineering, the production of industrially relevant chemicals, via rational
engineering of microorganisms, is an intensive area of research. One particular group of …

Bacteria deploy multiple defenses to prevent mobile genetic element (MGEs) invasion.
CRISPR–Cas immune systems use RNA-guided nucleases to target MGEs, which counter …

Transposition is a major mechanism of horizontal gene mobility in prokaryotes. However,
exploration of the genes mobilized by transposons (cargo) is hampered by the difficulty in …