Enhanced integration of large DNA into E. coli chromosome by CRISPR/Cas9
ME Chung, IH Yeh, LY Sung, MY Wu… - Biotechnology and …, 2017 - Wiley Online Library
Metabolic engineering often necessitates chromosomal integration of multiple genes but
integration of large genes into Escherichia coli remains difficult. CRISPR/Cas9 is an RNA …
integration of large genes into Escherichia coli remains difficult. CRISPR/Cas9 is an RNA …
CRISPR-Cas systems and the paradox of self-targeting spacers
F Wimmer, CL Beisel - Frontiers in microbiology, 2020 - frontiersin.org
CRISPR-Cas immune systems in bacteria and archaea record prior infections as spacers
within each system's CRISPR arrays. Spacers are normally derived from invasive genetic …
within each system's CRISPR arrays. Spacers are normally derived from invasive genetic …
It is unclear how important CRISPR-Cas systems are for protecting natural populations of bacteria against infections by mobile genetic elements
Articles on CRISPR commonly open with some variant of the phrase “these short
palindromic repeats and their associated endonucleases (Cas) are an adaptive immune …
palindromic repeats and their associated endonucleases (Cas) are an adaptive immune …
Inhibition of NHEJ repair by type II-A CRISPR-Cas systems in bacteria
A Bernheim, A Calvo-Villamañán, C Basier… - Nature …, 2017 - nature.com
Abstract Type II CRISPR-Cas systems introduce double-strand breaks into DNA of invading
genetic material and use DNA fragments to acquire novel spacers during adaptation. These …
genetic material and use DNA fragments to acquire novel spacers during adaptation. These …
Targeted-antibacterial-plasmids (TAPs) combining conjugation and CRISPR/Cas systems achieve strain-specific antibacterial activity
A Reuter, C Hilpert, A Dedieu-Berne… - Nucleic acids …, 2021 - academic.oup.com
The global emergence of drug-resistant bacteria leads to the loss of efficacy of our
antibiotics arsenal and severely limits the success of currently available treatments. Here …
antibiotics arsenal and severely limits the success of currently available treatments. Here …
Exploiting a conjugative CRISPR/Cas9 system to eliminate plasmid harbouring the mcr-1 gene from Escherichia coli
H Dong, H Xiang, D Mu, D Wang, T Wang - International journal of …, 2019 - Elsevier
The transfer of multi-drug-resistance plasmids by bacterial conjugation is largely responsible
for the development of drug resistance in bacteria, and causes serious problems in the …
for the development of drug resistance in bacteria, and causes serious problems in the …
Improved sgRNA design in bacteria via genome-wide activity profiling
CRISPR/Cas9 is a promising tool in prokaryotic genome engineering, but its success is
limited by the widely varying on-target activity of single guide RNAs (sgRNAs). Based on the …
limited by the widely varying on-target activity of single guide RNAs (sgRNAs). Based on the …
NT-CRISPR, combining natural transformation and CRISPR-Cas9 counterselection for markerless and scarless genome editing in Vibrio natriegens
The fast-growing bacterium Vibrio natriegens has recently gained increasing attention as a
novel chassis organism for fundamental research and biotechnology. To fully harness the …
novel chassis organism for fundamental research and biotechnology. To fully harness the …
Gene silencing with CRISPRi in bacteria and optimization of dCas9 expression levels
F Depardieu, D Bikard - Methods, 2020 - Elsevier
The catalytic null mutant of the Cas9 endonuclease from the bacterial CRISPR immune
system, known as dCas9, can be guided by a small RNA to bind DNA sequences of interest …
system, known as dCas9, can be guided by a small RNA to bind DNA sequences of interest …
Discovery and characterization of Cas9 inhibitors disseminated across seven bacterial phyla
CRISPR-Cas systems in bacteria and archaea provide immunity against bacteriophages
and plasmids. To overcome CRISPR immunity, phages have acquired anti-CRISPR genes …
and plasmids. To overcome CRISPR immunity, phages have acquired anti-CRISPR genes …