Gene-drive systems in diploid organisms bias the inheritance of one allele over another.
CRISPR-based gene-drive expresses a guide RNA (gRNA) into the genome at the site …

The catalytic null mutant of the Cas9 endonuclease from the bacterial CRISPR immune
system, known as dCas9, can be guided by a small RNA to bind DNA sequences of interest …

The fast-growing bacterium Vibrio natriegens has recently gained increasing attention as a
novel chassis organism for fundamental research and biotechnology. To fully harness the …

Leptospirosis is a neglected, widespread zoonosis caused by pathogenic species of the
genus Leptospira, and is responsible for 60,000 deaths per year. Pathogenic mechanisms of …

Background The exploitation of the CRISPR/Cas9 machinery coupled to lambda (λ)
recombinase-mediated homologous recombination (recombineering) is becoming the …

The development of antimicrobial-resistant (AMR) bacteria poses a serious worldwide
health concern. CRISPR-based antibacterials are a novel and adaptable method for …

Methanotrophic bacteria play a crucial role in the Earth's biogeochemical cycle and have the
potential to be employed in industrial biomanufacturing processes due to their capacity to …

Bacterial genome editing commonly relies on chromosomal cleavage with Cas nucleases to
counter-select against unedited cells. However, editing normally requires efficient …

The discovery of clustered, regularly interspaced, short palindromic repeats (CRISPR) and
the Cas9 RNA-guided nuclease provides unprecedented opportunities to selectively kill …

Mycoplasma species share a set of features, such as lack of a cell wall, streamlined
genomes, simplified metabolism, and the use of a deviant genetic code, that make them …