Biocatalysis has emerged as a promising alternative to conventional chemical processes for
the production of a wide range of chemicals, providing a sustainable solution to the problem …

ABSTRACT CRISPR-Cas systems provide adaptive immunity against phages in prokaryotes
via DNA-encoded, RNA-mediated, nuclease-dependent targeting and cleavage. Due to …

During infection, phages manipulate bacteria to redirect metabolism towards viral
proliferation. To counteract phages, some bacteria employ CRISPR-Cas systems that …

Phage-derived “recombineering” methods are utilized for bacterial genome editing.
Recombineering results in a heterogeneous population of modified and unmodified …

CRISPR-Cas provides a barrier to horizontal gene transfer in prokaryotes. It was previously
observed that functional CRISPR-Cas systems are absent from multidrug-resistant (MDR) …

Highlights•LAB genomics revolution revived interest in (traditional) genetic mobilization
methodologies.•Natural competence is principally not constrained by strain-compatibility …

ABSTRACT CRISPR-Cas9 technology has been exploited for the removal or replacement of
genetic elements in a wide range of prokaryotes and eukaryotes. Here, we describe the …

Streptococcussuis is an important zoonotic pathogen that causes severe invasive disease in
pigs and humans. Current methods for genome engineering of S. suis rely on the insertion of …

Deep mutational scanning can provide significant insights into the function of essential
genes in bacteria. Here, we developed a high‐throughput method for mutating essential …

Cupriavidus nantongensis X1T is a type strain of the genus Cupriavidus, that can degrade
eight kinds of organophosphorus insecticides (OPs). Conventional genetic manipulations in …