Bacterial systems for production of heterologous proteins

S Zerbs, AM Frank, FR Collart - Methods in enzymology, 2009 - Elsevier
Proteins are the working molecules of all biological systems and participate in a majority of
cellular chemical reactions and biological processes. Knowledge of the properties and …

Laue crystal structure of Shewanella oneidensis cytochrome c nitrite reductase from a high-yield expression system

M Youngblut, ET Judd, V Srajer, B Sayyed… - JBIC Journal of …, 2012 - Springer
The high-yield expression and purification of Shewanella oneidensis cytochrome c nitrite
reductase (ccNiR) and its characterization by a variety of methods, notably Laue …

Interaction studies between periplasmic cytochromes provide insights into extracellular electron transfer pathways of Geobacter sulfurreducens

AP Fernandes, TC Nunes, CM Paquete… - Biochemical …, 2017 - portlandpress.com
Geobacter bacteria usually prevail among other microorganisms in soils and sediments
where Fe (III) reduction has a central role. This reduction is achieved by extracellular …

pSW2, a novel low-temperature-inducible gene expression vector based on a filamentous phage of the deep-sea bacterium Shewanella piezotolerans WP3

XW Yang, HH Jian, FP Wang - Applied and Environmental …, 2015 - Am Soc Microbiol
ABSTRACT A low-temperature-inducible protein expression vector (pSW2) based on a
filamentous phage (SW1) of the deep-sea bacterium Shewanella piezotolerans WP3 was …

Heterologous expression and purification of a multiheme cytochrome from a Gram-positive bacterium capable of performing extracellular respiration

NL Costa, HK Carlson, JD Coates, RO Louro… - Protein Expression and …, 2015 - Elsevier
Microbial electrochemical technologies are emerging as environmentally friendly
biotechnological processes. Recently, a thermophilic Gram-positive bacterium capable of …

在大肠杆菌周质表达重组蛋白的研究进展

李振国, 徐明波, 牛罡, 陈遥, 姚文兵 - 药物生物技术, 2011 - cqvip.com
大肠杆菌表达系统是基因表达技术中发展最早和应用最广的表达系统, 由于大肠杆菌周质空间
具有其独特优势, 重组蛋白在周质表达和提取技术得到国内外学者的关注 …

Characterization of a cryptic plasmid pSFKW33 from Shewanella sp. 33B

K Werbowy, H Cieśliński, J Kur - Plasmid, 2009 - Elsevier
A cryptic plasmid pSFKW33 from psychrotrophic bacterium Shewanella sp. 33B, an isolate
from the Gulf of Gdansk (the Baltic Sea), was sequenced and characterized. It is an 8021bp …

Functional expression of Carassius auratus cytochrome P4501A in a novel Shewanella oneidensis expression system and application for the degradation of benzo [a] …

Z Chang, M Lu, KJ Shon, JS Park - Journal of Biotechnology, 2014 - Elsevier
Abstract Cytochrome P4501A (CYP1A) in fish has attracted an increasing interest because
of its important roles in the metabolic activation of certain xenobiotics such as aromatic …

大肠杆菌周质蛋白提取工艺的研究

李振国, 徐明波, 牛罡, 姚文兵 - 生物学杂志, 2011 - cqvip.com
研究了精氨酸缓冲液在不同浓度, pH 值及提取时间下的周质蛋白提取率, 并以溶菌酶法,
渗透压休克法作为对比. 结果表明浓度0.4 mol/L, pH 值8.0, 提取时间为45min 时 …

The antiangiogenic activity of a soluble fragment of the VEGFR extracellular domain

H Li, W Cao, Z Chen, DO Acheampong, H Jin… - Biomedicine & …, 2013 - Elsevier
Vascular endothelial growth factor (VEGF) is a key regulator of pathological angiogenesis
and vascular permeability and overexpressed by most solid tumors. VEGF receptor-2 …