[HTML][HTML] 非限制性内切核酸酶Sma 的表达纯化工艺及性能研究
徐一帆， 刘明秋 - 中国生物工程杂志, 2017 - html.rhhz.net
目的大量表达非限制性内切核酸酶Sma 并获得高纯度目的蛋白, 对其酶活进行鉴定. 方法PCR
获得Sma 基因片段, 构建pET28a-ompA-Sma 表达载体, 转入E. coli BL21 (DE3) 中 …
获得Sma 基因片段, 构建pET28a-ompA-Sma 表达载体, 转入E. coli BL21 (DE3) 中 …
[HTML][HTML] 纳米抗体异源表达的研究进展
李丹， 黄鹤 - 中国生物工程杂志, 2017 - html.rhhz.net
羊驼体内存在天然缺失轻链的重链抗体(HcAb), 其单域抗原结合片段也称为VHH
或纳米抗体(nanobody, Nb), 是目前已知的最小抗原结合片段. 与传统抗体相比 …
或纳米抗体(nanobody, Nb), 是目前已知的最小抗原结合片段. 与传统抗体相比 …
类弹性蛋白标签在大肠杆菌周质空间中表达研究
李晶泉， 林衡， 丁宁， 左晓雪， 史晋萍， 徐永平… - 生物技术 …, 2016 - biotech.aiijournal.com
旨在研究类弹性蛋白[I] 40 (elastin-like polypeptide [I] 40, ELP [I] 40) 在大肠杆菌周质空间的
表达. 构建表达载体pIG6LH/ELP [I] 40 及pIG6LH/ELP [I] 40+ Trx, 分别将构建的表达载体转化入 …
表达. 构建表达载体pIG6LH/ELP [I] 40 及pIG6LH/ELP [I] 40+ Trx, 分别将构建的表达载体转化入 …
ИССЛЕДОВАНИЕ Sec-СИСТЕМЫ ТРАНСЛОКАЦИИ БЕЛКОВ ПРИ ГЕТЕРОЛОГИЧНОЙ ЭКСПРЕССИИ ГЕНОВ В КЛЕТКАХ БАКТЕРИИ Shewanella oneidensis …
НН Мордкович, НА Окорокова, ВП Вейко - Прикладная биохимия и …, 2015 - elibrary.ru
Проведено сравнение первичных структур белков Sec-системы транслокации белков у
бактерий Shewanella oneidensis MR-1 и Escherichia coli. Изучен процесс транслокации …
бактерий Shewanella oneidensis MR-1 и Escherichia coli. Изучен процесс транслокации …
Living capacitors: functional characterization of a novel cytochrome acting as a nanowire
APN Fernandes - 2019 - search.proquest.com
In an Era where environmental issues are a growing concern, microorganisms that have
remarkable features, such as extracellular electron transfer (EET) ability, present major …
remarkable features, such as extracellular electron transfer (EET) ability, present major …
Investigation of protein translocation Sec-system with heterologous gene expression in Shewanella oneidensis MR-1 bacterium cells
NN Mordkovich, NA Okorokova, VP Veiko - Applied biochemistry and …, 2015 - Springer
A comparison of the primary structures of the protein translocation Sec-system proteins in
the Shewanella oneidensis MR-1 and Escherichia coli bacteria was carried out. The process …
the Shewanella oneidensis MR-1 and Escherichia coli bacteria was carried out. The process …
Expression and Purification Procedure of Nonspecific Endonuclease Sma and Its Performance Study
Y XU, M LIU - China Biotechnology, 2017 - manu60.magtech.com.cn
Objective: To express nonspecific endonuclease Serratia marcescens (Sma), gain the high
purity expressed product, and determine its activity. Methods: Sma fragment was produced …
purity expressed product, and determine its activity. Methods: Sma fragment was produced …
Heterologous Expression of Nanobodies: a Recent Progress
D LI, H Huang - China Biotechnology, 2017 - manu60.magtech.com.cn
Heavy chain antibodies (HcAb) without light chains are naturally produced by camelids. The
single domain antigen-binding fragment of HcAb is referred to as VHH or nanobody (Nb) …
single domain antigen-binding fragment of HcAb is referred to as VHH or nanobody (Nb) …
The Expression of Elastin-like Polypeptide Tag in Periplasmic Space of Escherichia coli
J LI, H LIN, N DING, X ZUO, J SHI, Y XU… - Biotechnology …, 2016 - biotech.aiijournal.com
This work aims to investigate the expression of elastin-like polypeptide [I] 40 (ELP [I] 40) in
the periplasmic space of Escherichia coli. The expression vectors of pIG6LH/ELP [I] 40 and …
the periplasmic space of Escherichia coli. The expression vectors of pIG6LH/ELP [I] 40 and …
[PDF][PDF] Федеральное государственное унитарное предприятие Государственный научно-исследовательский институт генетики и селекции промышленных …
НН Мордкович - 2014 - genetika.ru
Способность определенных групп бактерий к восстановлению металлов в процессе
роста и анаэробного дыхания делает их все более актуальными для использования как …
роста и анаэробного дыхания делает их все более актуальными для использования как …